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Deprecated: Implicit conversion from float 294.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Transfusion 2014 ; 54 (4): 1059-70 Nephropedia Template TP
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Mononuclear cells from a rare blood donor, after freezing under good manufacturing practice conditions, generate red blood cells that recapitulate the rare blood phenotype #MMPMID24004289
Masiello F; Tirelli V; Sanchez M; van den Akker E; Girelli G; Marconi M; Villa MA; Rebulla P; Hashmi G; Whitsett C; Migliaccio AR
Transfusion 2014[Apr]; 54 (4): 1059-70 PMID24004289show ga
Background: Cultured red blood cells (cRBCs) from cord blood (CB) have been proposed as transfusion products. Whether buffy-coats discarded from blood donations (AB) may be used to generate cRBCs for transfusion has not been investigated. Study Design and Methods: Erythroid progenitor cell content and numbers and blood group antigen profiles of erythroblasts (ERYs) and cRBCs generated in Human Erythroid Massive Amplification (HEMA) culture by CB (n=7) and AB (n=33, three females, three males, one AB with rare blood antigens cryopreserved using CB protocols) were compared. Results: Variability was observed both in progenitor cell content (2-fold) and number of ERYs generated (1-log) by CB and AB in HEMA. The average progenitor cell contents of the subset of AB and CB analyzed were similar. AB generated numbers of ERYs 3-times lower (p<0.01) than CB in HEMA containing fetal bovine serum but similar to CB in HEMA containing human proteins. Female AB contained 2-times less (p<0.05) erythroid progenitor cells but generated numbers of ERYs similar to those generated by male AB. Cryopreserved AB with a rare blood group phenotype and shipped to another laboratory generated great numbers of ERYs, 90% of which matured into cRBCs. Blood group antigen expression was consistent with the donor genotype for ERYs generated both by CB and AB but concordant with that of native RBCs only for cells derived from AB. Conclusion: Buffy-coats from regular donors, including a donor with rare phenotypes stored under conditions established for CB, are not inferior to CB for the generation of cRBCs.