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1985 ; 82
(23
): 8208-12
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Human monocyte-endothelial cell interaction in vitro
#MMPMID3865221
Pawlowski NA
; Abraham EL
; Pontier S
; Scott WA
; Cohn ZA
Proc Natl Acad Sci U S A
1985[Dec]; 82
(23
): 8208-12
PMID3865221
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We have examined the interaction of freshly isolated human blood monocytes with
cultured human umbilical vein endothelial cells in vitro. Purified monocytes
incubated with confluent primary or passaged endothelial cells (EC) for 90 min at
37 degrees C bound at maximal densities of 6.5-7.0 X 10(3)/mm2 (8 or 9 per EC)
without causing disruption of the monolayer. Monocyte-EC binding proceeded in the
presence of plasma proteins or optimal phagocytic doses of opsonized zymosan
particles. The avidity of attachment was not diminished by alternative monocyte
isolation techniques. Monocyte attachment to EC was dependent upon the presence
of divalent cations (magnesium greater than calcium) and was inhibited at 4
degrees C. Monocytes selectively bound to EC when incubated with monolayers
composed of smooth muscle cells and EC. Neither EC monolayer confluence nor a
variety of EC culture conditions affected the high levels of monocyte binding. In
contrast, human neutrophils (less than 1 per EC) and lymphocytes (less than 2-3.5
per EC) bound at lower maximal densities under the same conditions, while
platelet reactivity remained minimal. The distinctively higher affinity of human
blood monocytes relative to other circulating white cells for binding to cultured
human EC may have relevance to their function in vivo.
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