Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1128/AEM.02184-13 http://scihub22266oqcxt.onion/10.1128/AEM.02184-13 C3837754!3837754!24056460     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Appl+Environ+Microbiol 2013 ; 79 (23): 7390-7 Nephropedia Template TP {{tp|p=24056460|t=2013. Survival of Bactericidal Antibiotic Treatment by a Persister Subpopulation of Listeria monocytogenes |pdf=|usr=}}{{24056460}} gab.com Text Survival of Bactericidal Antibiotic Treatment by a Persister Subpopulation of Listeria monocytogenes JO: Appl Environ Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=24056460 #FOAvip Listeria monocytogenes can cause the serious infection listeriosis, which despite antibiotic treatment has a high mortality. Understanding the response of L. monocytogenes to antibiotic exposure is therefore important to ensure treatment success. Some bacteria survive antibiotic treatment by formation of persisters, which are a dormant antibiotic-tolerant subpopulation. The purpose of this study was to determine whether L. monocytogenes can form persisters and how bacterial physiology affects the number of persisters in the population. A stationary-phase culture of L. monocytogenes was adjusted to 108 CFU ml?1, and 103 to 104 CFU ml?1 survived 72-h treatment with 100 ?g of norfloxacin ml?1, indicating a persister subpopulation. This survival was not caused by antibiotic resistance as regrown persisters were as sensitive to norfloxacin as the parental strain. Higher numbers of persisters (105 to 106) were surviving when older stationary phase or surface-associated cells were treated with 100 ?g of norfloxacin ml?1. The number of persisters was similar when a ?sigB mutant and the wild type were treated with norfloxacin, but the killing rate was higher in the ?sigB mutant. Dormant norfloxacin persisters could be activated by the addition of fermentable carbohydrates and subsequently killed by gentamicin; however, a stable surviving subpopulation of 103 CFU ml?1 remained. Nitrofurantoin that has a growth-independent mode of action was effective against both growing and dormant cells, suggesting that eradication of persisters is possible. Our study adds L. monocytogenes to the list of bacterial species capable of surviving bactericidal antibiotics in a dormant stage, and this persister phenomenon should be borne in mind when developing treatment regimens. Twit Text FOAVip Survival of Bactericidal Antibiotic Treatment by a Persister Subpopulation of Listeria monocytogenes ????Appl Environ Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=24056460 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24056460 #FOAvip English Wikipedia <ref>{{Cite pmid|24056460}}</ref> Survival of Bactericidal Antibiotic Treatment by a Persister Subpopulation of Listeria monocytogenes #MMPMID24056460 Knudsen GM; Ng Y; Gram L Appl Environ Microbiol 2013[Dec]; 79 (23): 7390-7 PMID24056460show ga Listeria monocytogenes can cause the serious infection listeriosis, which despite antibiotic treatment has a high mortality. Understanding the response of L. monocytogenes to antibiotic exposure is therefore important to ensure treatment success. Some bacteria survive antibiotic treatment by formation of persisters, which are a dormant antibiotic-tolerant subpopulation. The purpose of this study was to determine whether L. monocytogenes can form persisters and how bacterial physiology affects the number of persisters in the population. A stationary-phase culture of L. monocytogenes was adjusted to 108 CFU ml?1, and 103 to 104 CFU ml?1 survived 72-h treatment with 100 ?g of norfloxacin ml?1, indicating a persister subpopulation. This survival was not caused by antibiotic resistance as regrown persisters were as sensitive to norfloxacin as the parental strain. Higher numbers of persisters (105 to 106) were surviving when older stationary phase or surface-associated cells were treated with 100 ?g of norfloxacin ml?1. The number of persisters was similar when a ?sigB mutant and the wild type were treated with norfloxacin, but the killing rate was higher in the ?sigB mutant. Dormant norfloxacin persisters could be activated by the addition of fermentable carbohydrates and subsequently killed by gentamicin; however, a stable surviving subpopulation of 103 CFU ml?1 remained. Nitrofurantoin that has a growth-independent mode of action was effective against both growing and dormant cells, suggesting that eradication of persisters is possible. Our study adds L. monocytogenes to the list of bacterial species capable of surviving bactericidal antibiotics in a dormant stage, and this persister phenomenon should be borne in mind when developing treatment regimens. äSurvival of Bactericidal Antibiotic Treatment by a Persister Subpopula(epmc).pdf DeepDyve Pubget Overpricing