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2013 ; 79
(23
): 7390-7
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Survival of bactericidal antibiotic treatment by a persister subpopulation of
Listeria monocytogenes
#MMPMID24056460
Knudsen GM
; Ng Y
; Gram L
Appl Environ Microbiol
2013[Dec]; 79
(23
): 7390-7
PMID24056460
show ga
Listeria monocytogenes can cause the serious infection listeriosis, which despite
antibiotic treatment has a high mortality. Understanding the response of L.
monocytogenes to antibiotic exposure is therefore important to ensure treatment
success. Some bacteria survive antibiotic treatment by formation of persisters,
which are a dormant antibiotic-tolerant subpopulation. The purpose of this study
was to determine whether L. monocytogenes can form persisters and how bacterial
physiology affects the number of persisters in the population. A stationary-phase
culture of L. monocytogenes was adjusted to 10(8) CFU ml(-1), and 10(3) to 10(4)
CFU ml(-1) survived 72-h treatment with 100 ?g of norfloxacin ml(-1), indicating
a persister subpopulation. This survival was not caused by antibiotic resistance
as regrown persisters were as sensitive to norfloxacin as the parental strain.
Higher numbers of persisters (10(5) to 10(6)) were surviving when older
stationary phase or surface-associated cells were treated with 100 ?g of
norfloxacin ml(-1). The number of persisters was similar when a ?sigB mutant and
the wild type were treated with norfloxacin, but the killing rate was higher in
the ?sigB mutant. Dormant norfloxacin persisters could be activated by the
addition of fermentable carbohydrates and subsequently killed by gentamicin;
however, a stable surviving subpopulation of 10(3) CFU ml(-1) remained.
Nitrofurantoin that has a growth-independent mode of action was effective against
both growing and dormant cells, suggesting that eradication of persisters is
possible. Our study adds L. monocytogenes to the list of bacterial species
capable of surviving bactericidal antibiotics in a dormant stage, and this
persister phenomenon should be borne in mind when developing treatment regimens.
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