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10.1111/j.1582-4934.2009.00780.x http://scihub22266oqcxt.onion/10.1111/j.1582-4934.2009.00780.x C3828850!3828850 !19432813     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\19432813 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Cell+Mol+Med 2010 ; 14 (6A ): 1338-46 Nephropedia Template TP {{tp|p=19432813 |t=2010. Mechanical load modulates chondrogenesis of human mesenchymal stem cells through the TGF-beta pathway |pdf=|usr=}}{{19432813 }} gab.com Text Mechanical load modulates chondrogenesis of human mesenchymal stem cells through the TGF-beta pathway JO: J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=19432813 #FOAvip This study investigated the effect of mechanical load on human mesenchymal stem cell (hMSC) differentiation under different exogenous transforming growth factor-beta1 (TGF-beta(1)) concentrations (0, 1 or 10 ng/ml).The role of the TGF-beta signalling pathway in this process was also studied. Human MSCs were seeded into fibrin-biodegradable polyurethane scaffolds at a cell density of 5 x 10(6) cells per scaffold and stimulated using our bioreactor. One hour of surface motion superimposed on cyclic compression was applied once a day over seven consecutive days. Scaffolds were analysed for gene expression, DNA content and glycosaminoglycan amount. Addition of TGF-beta(1) in the culture medium was sufficient to induce chondrogenesis of hMSCs. Depending on the TGF-beta(1) concentration of the culture medium, mechanical load stimulated chondrogenesis of hMSCs compared to the unloaded scaffolds, with a much stronger effect on gene expression at lower TGF-beta(1) concentrations. With TGF-beta(1) absent in the culture medium, mechanical load stimulated gene transcripts and protein synthesis of TGF-beta(1) and TGF-beta(3). TGF-beta type I receptor inhibitor LY364947 blocked the up-regulation on TGF-beta(1) and TGF-beta(3) production stimulated by mechanical load, and also blocked the chondrogenesis of hMSCs. Taken together, these findings suggest that mechanical load promotes chondrogenesis of hMSCs through TGF-beta pathway by up-regulating TGF-beta gene expression and protein synthesis. Twit Text FOAVip Mechanical load modulates chondrogenesis of human mesenchymal stem cells through the TGF-beta pathway ????J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=19432813 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=19432813 #FOAvip English Wikipedia <ref>{{Cite pmid|19432813 }}</ref> Mechanical load modulates chondrogenesis of human mesenchymal stem cells through the TGF-beta pathway #MMPMID19432813 Li Z ; Kupcsik L ; Yao SJ ; Alini M ; Stoddart MJ J Cell Mol Med 2010[Jun]; 14 (6A ): 1338-46 PMID19432813 show ga This study investigated the effect of mechanical load on human mesenchymal stem cell (hMSC) differentiation under different exogenous transforming growth factor-beta1 (TGF-beta(1)) concentrations (0, 1 or 10 ng/ml).The role of the TGF-beta signalling pathway in this process was also studied. Human MSCs were seeded into fibrin-biodegradable polyurethane scaffolds at a cell density of 5 x 10(6) cells per scaffold and stimulated using our bioreactor. One hour of surface motion superimposed on cyclic compression was applied once a day over seven consecutive days. Scaffolds were analysed for gene expression, DNA content and glycosaminoglycan amount. Addition of TGF-beta(1) in the culture medium was sufficient to induce chondrogenesis of hMSCs. Depending on the TGF-beta(1) concentration of the culture medium, mechanical load stimulated chondrogenesis of hMSCs compared to the unloaded scaffolds, with a much stronger effect on gene expression at lower TGF-beta(1) concentrations. With TGF-beta(1) absent in the culture medium, mechanical load stimulated gene transcripts and protein synthesis of TGF-beta(1) and TGF-beta(3). TGF-beta type I receptor inhibitor LY364947 blocked the up-regulation on TGF-beta(1) and TGF-beta(3) production stimulated by mechanical load, and also blocked the chondrogenesis of hMSCs. Taken together, these findings suggest that mechanical load promotes chondrogenesis of hMSCs through TGF-beta pathway by up-regulating TGF-beta gene expression and protein synthesis. |*Stress, Mechanical [MESH] |Cells, Cultured [MESH] |Chondrogenesis/*drug effects/genetics [MESH] |Culture Media, Conditioned/pharmacology [MESH] |DNA/metabolism [MESH] |Enzyme-Linked Immunosorbent Assay [MESH] |Gene Expression Regulation/drug effects [MESH] |Glycosaminoglycans/metabolism [MESH] |Humans [MESH] |Mesenchymal Stem Cells/*drug effects/*metabolism [MESH] |Protein Serine-Threonine Kinases/antagonists & inhibitors/metabolism [MESH] |Receptor, Transforming Growth Factor-beta Type I [MESH] |Receptors, Transforming Growth Factor beta/antagonists & inhibitors/metabolism [MESH] |Signal Transduction/*drug effects [MESH] |Tissue Scaffolds [MESH] |Transforming Growth Factor beta1/*pharmacology [MESH]Mechanical load modulates chondrogenesis of human mesenchymal stem cel(epmc).pdf DeepDyve Pubget Overpricing