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10.1111/j.1582-4934.2009.00756.x http://scihub22266oqcxt.onion/10.1111/j.1582-4934.2009.00756.x C3822883!3822883!19382907     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Cell+Mol+Med 2009 ; 13 (4): 771-7 Nephropedia Template TP {{tp|p=19382907|t=2009. Epicardium: interstitial Cajal-like cells (ICLC) highlighted by immunofluorescence |pdf=|usr=}}{{19382907}} gab.com Text Epicardium: interstitial Cajal-like cells (ICLC) highlighted by immunofluorescence JO: J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=19382907 #FOAvip During the last few years, there is an increasing interest in the role of the epicardium in cardiac development, myocardial remodelling or repair and regeneration. Several types of cells were described in the subepicardial loose connective tissue, beneath the epicardial mesothe-lium. We showed previously (repeatedly) the existence of interstitial Cajal-like cells (ICLCs) in human and mammalian myocardium, either in atria or in ventricles. Here, we describe ICLCs in adult mice epicardium and primary culture as well as in situ using frozen sections. The identification of ICLCs was based on phase contrast microscopy and immunophenotyping. We found cells with characteristic morphologic aspects: spindle-shaped, triangular or polygonal cell body and typical very long (tens to hundreds micrometres) and very thin cyto-plasmic processes, with a distinctive ?beads-on-a-string? appearance. The dilations contain mitochondria, as demonstrated by MitoTracker Green FM labelling of living cells. Epicardial ICLCs were found positive for c-kit/CD117 and/or CD34. However, we also observed ICLCs positive for c-kit and vimentin. In conclusion, ICLCs represent a distinct cell type in the subendocardium, presumably comprising at least two subpopulations: (i) c-kit/CD34-positive and (ii) only c-kit-positive. ICLCs might be essential as progenitor (or promoter) cells for developing cardiomyocyte lineages in normal and/or injured heart. Twit Text FOAVip Epicardium: interstitial Cajal-like cells (ICLC) highlighted by immunofluorescence ????J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=19382907 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=19382907 #FOAvip English Wikipedia <ref>{{Cite pmid|19382907}}</ref> Epicardium: interstitial Cajal-like cells (ICLC) highlighted by immunofluorescence #MMPMID19382907 Suciu L; Popescu LM; Regalia T; Ardelean A; Manole CG J Cell Mol Med 2009[Apr]; 13 (4): 771-7 PMID19382907show ga During the last few years, there is an increasing interest in the role of the epicardium in cardiac development, myocardial remodelling or repair and regeneration. Several types of cells were described in the subepicardial loose connective tissue, beneath the epicardial mesothe-lium. We showed previously (repeatedly) the existence of interstitial Cajal-like cells (ICLCs) in human and mammalian myocardium, either in atria or in ventricles. Here, we describe ICLCs in adult mice epicardium and primary culture as well as in situ using frozen sections. The identification of ICLCs was based on phase contrast microscopy and immunophenotyping. We found cells with characteristic morphologic aspects: spindle-shaped, triangular or polygonal cell body and typical very long (tens to hundreds micrometres) and very thin cyto-plasmic processes, with a distinctive ?beads-on-a-string? appearance. The dilations contain mitochondria, as demonstrated by MitoTracker Green FM labelling of living cells. Epicardial ICLCs were found positive for c-kit/CD117 and/or CD34. However, we also observed ICLCs positive for c-kit and vimentin. In conclusion, ICLCs represent a distinct cell type in the subendocardium, presumably comprising at least two subpopulations: (i) c-kit/CD34-positive and (ii) only c-kit-positive. ICLCs might be essential as progenitor (or promoter) cells for developing cardiomyocyte lineages in normal and/or injured heart. äEpicardium: interstitial Cajal-like cells (ICLC) highlighted by immuno(epmc).pdf DeepDyve Pubget Overpricing