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Ferroportin 1 is expressed basolaterally in rat kidney proximal tubule cells and
iron excess increases its membrane trafficking
#MMPMID20015204
Wolff NA
; Liu W
; Fenton RA
; Lee WK
; Thévenod F
; Smith CP
J Cell Mol Med
2011[Feb]; 15
(2
): 209-19
PMID20015204
show ga
Ferroportin 1 (FPN1) is an iron export protein expressed in liver and duodenum,
as well as in reticuloendothelial macrophages. Previously, we have shown that
divalent metal transporter 1 (DMT1) is expressed in late endosomes and lysosomes
of the kidney proximal tubule (PT), the nephron segment responsible for the
majority of solute reabsorption. We suggested that following receptor mediated
endocytosis of transferrin filtered by the glomerulus, DMT1 exports iron
liberated from transferrin into the cytosol. FPN1 is also expressed in the kidney
yet its role remains obscure. As a first step towards determining the role of
renal FPN1, we localized FPN1 in the PT. FPN1 was found to be located in
association with the basolateral PT membrane and within the cytosolic
compartment. FPN1 was not expressed on the apical brush-border membrane of PT
cells. These data support a role for FPN1 in vectorial export of iron out of PT
cells. Furthermore, under conditions of iron loading of cultured PT cells, FPN1
was trafficked to the plasma membrane suggesting a coordinated cellular response
to export excess iron and limit cellular iron concentrations.
|Animals
[MESH]
|Cation Transport Proteins/biosynthesis/genetics/*metabolism
[MESH]
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