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10.1016/j.cell.2013.05.006 http://scihub22266oqcxt.onion/10.1016/j.cell.2013.05.006 C3772789!3772789!23683578     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell 2013 ; 153 (6): 1228-38 Nephropedia Template TP {{tp|p=23683578|t=2013. Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer |pdf=|usr=}}{{23683578}} gab.com Text Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=23683578 #FOAvip Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an approach for generating patient-matched nuclear transfer (NT)-ESCs for studies of disease mechanisms and for developing specific therapies. Past attempts to produce human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryos. Here, we identified premature exit from meiosis in human oocytes and suboptimal activation as key factors that are responsible for these outcomes. Optimized SCNT approaches designed to circumvent these limitations allowed derivation of human NT-ESCs. When applied to premium quality human oocytes, NT-ESC lines were derived from as few as two oocytes. NT-ESCs displayed normal diploid karyotypes and inherited their nuclear genome exclusively from parental somatic cells. Gene expression and differentiation profiles in human NT-ESCs were similar to embryo-derived ESCs, suggesting efficient reprogramming of somatic cells to a pluripotent state. Twit Text FOAVip Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=23683578 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23683578 #FOAvip English Wikipedia <ref>{{Cite pmid|23683578}}</ref> Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer #MMPMID23683578 Tachibana M; Amato P; Sparman M; Gutierrez NM; Tippner-Hedges R; Ma H; Kang E; Fulati A; Lee HS; Sritanaudomchai H; Masterson K; Larson J; Eaton D; Sadler-Fredd K; Battaglia D; Lee D; Wu D; Jensen J; Patton P; Gokhale S; Stouffer RL; Wolf D; Mitalipov S Cell 2013[Jun]; 153 (6): 1228-38 PMID23683578show ga Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an approach for generating patient-matched nuclear transfer (NT)-ESCs for studies of disease mechanisms and for developing specific therapies. Past attempts to produce human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryos. Here, we identified premature exit from meiosis in human oocytes and suboptimal activation as key factors that are responsible for these outcomes. Optimized SCNT approaches designed to circumvent these limitations allowed derivation of human NT-ESCs. When applied to premium quality human oocytes, NT-ESC lines were derived from as few as two oocytes. NT-ESCs displayed normal diploid karyotypes and inherited their nuclear genome exclusively from parental somatic cells. Gene expression and differentiation profiles in human NT-ESCs were similar to embryo-derived ESCs, suggesting efficient reprogramming of somatic cells to a pluripotent state. äHuman Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer (epmc).pdf DeepDyve Pubget Overpricing