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10.1007/s00592-013-0479-5 http://scihub22266oqcxt.onion/10.1007/s00592-013-0479-5 C3770827!3770827!23624551     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=23624551&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Acta+Diabetol 2014 ; 51 (2): 199-204 Nephropedia Template TP {{tp|p=23624551|t=2014. Gene Expression Analysis of Human Islets in a Subject at Onset of Type 1 Diabetes |pdf=|usr=}}{{23624551}} gab.com Text Gene Expression Analysis of Human Islets in a Subject at Onset of Type 1 Diabetes JO: Acta Diabetol http://www.kidney.de/pget.php?&tw=1&pmid=23624551 #FOAvip Swollen islet cells have been repeatedly described at onset of type 1 diabetes, but the underlying mechanism of this observation, termed hydropic degeneration, awaits characterization. In this study, laser capture microdissection was applied to extract the islets from an organ donor that died at onset of type 1 diabetes and from an organ donor without pancreatic disease. Morphologic analysis revealed extensive hydropic degeneration in 73% of the islets from the donor with type 1 diabetes. Expression levels of genes involved in apoptosis, ER stress, beta cell function, and inflammation were analyzed in isolated and laser-captured islets by qPCR. The chemokine MCP-1 was expressed in islets from the donor with type 1 diabetes while undetectable in the control donor. No other signs of inflammation were detected. There were no signs of apoptosis on the gene expression level, which was also confirmed by negative immunostaining for cleaved caspase-8. There was an increased expression of the transcription factor ATF4, involved in transcription of ER stress genes, in the diabetic islets but no further signs of ER stress were identified.In summary, on the transcription level, islets at onset of type 1 diabetes in which many beta cells display hydropic degeneration show no obvious signs of apoptosis, ER stress or inflammation supporting the notion that these cells are responding normally to high glucose and eventually succumbing in beta cell exhaustion. Also, this study validates the feasibility of performing qPCR analysis of RNA extracted from islets from subjects with recent onset T1D and healthy controls by laser capture microdissection. Twit Text FOAVip Gene Expression Analysis of Human Islets in a Subject at Onset of Type 1 Diabetes ????Acta Diabetol http://www.kidney.de/pget.php?&tw=1&pmid=23624551 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23624551 #FOAvip English Wikipedia <ref>{{Cite pmid|23624551}}</ref> Gene Expression Analysis of Human Islets in a Subject at Onset of Type 1 Diabetes #MMPMID23624551 Hopfgarten J; Stenwall PA; Wiberg A; Anagandula M; Ingvast S; Rosenling T; Korsgren O; Skog O Acta Diabetol 2014[Apr]; 51 (2): 199-204 PMID23624551show ga Swollen islet cells have been repeatedly described at onset of type 1 diabetes, but the underlying mechanism of this observation, termed hydropic degeneration, awaits characterization. In this study, laser capture microdissection was applied to extract the islets from an organ donor that died at onset of type 1 diabetes and from an organ donor without pancreatic disease. Morphologic analysis revealed extensive hydropic degeneration in 73% of the islets from the donor with type 1 diabetes. Expression levels of genes involved in apoptosis, ER stress, beta cell function, and inflammation were analyzed in isolated and laser-captured islets by qPCR. The chemokine MCP-1 was expressed in islets from the donor with type 1 diabetes while undetectable in the control donor. No other signs of inflammation were detected. There were no signs of apoptosis on the gene expression level, which was also confirmed by negative immunostaining for cleaved caspase-8. There was an increased expression of the transcription factor ATF4, involved in transcription of ER stress genes, in the diabetic islets but no further signs of ER stress were identified.In summary, on the transcription level, islets at onset of type 1 diabetes in which many beta cells display hydropic degeneration show no obvious signs of apoptosis, ER stress or inflammation supporting the notion that these cells are responding normally to high glucose and eventually succumbing in beta cell exhaustion. Also, this study validates the feasibility of performing qPCR analysis of RNA extracted from islets from subjects with recent onset T1D and healthy controls by laser capture microdissection. äGene Expression Analysis of Human Islets in a Subject at Onset of Type(epmc).pdf DeepDyve Pubget Overpricing