Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=23945047
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\23945047
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 AMB+Express
2013 ; 3
(1
): 45
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Overexpression of an antimicrobial peptide derived from C elegans using an
aggregation-prone protein coexpression system
#MMPMID23945047
Tomisawa S
; Hojo E
; Umetsu Y
; Ohki S
; Kato Y
; Miyazawa M
; Mizuguchi M
; Kamiya M
; Kumaki Y
; Kikukawa T
; Kawano K
; Demura M
; Aizawa T
AMB Express
2013[Aug]; 3
(1
): 45
PMID23945047
show ga
Antibacterial factor 2 (ABF-2) is a 67-residue antimicrobial peptide derived from
the nematode Caenorhabditis elegans. Although it has been reported that ABF-2
exerts in vitro microbicidal activity against a range of bacteria and fungi, the
structure of ABF-2 has not yet been solved. To enable structural studies of ABF-2
by NMR spectroscopy, a large amount of isotopically labeled ABF-2 is essential.
However, the direct expression of ABF-2 in Escherichia coli is difficult to
achieve due to its instability. Therefore, we applied a coexpression method to
the production of ABF-2 in order to enhance the inclusion body formation of
ABF-2. The inclusion body formation of ABF-2 was vastly enhanced by coexpression
of aggregation-prone proteins (partner proteins). By using this method, we
succeeded in obtaining milligram quantities of active, correctly folded ABF-2. In
addition, 15?N-labeled ABF-2 and a well-dispersed heteronuclear single quantum
coherence (HSQC) spectrum were also obtained successfully. Moreover, the effect
of the charge of the partner protein on the inclusion body formation of ABF-2 in
this method was investigated by using four structurally homologous proteins. We
concluded that a partner protein of opposite charge enhanced the formation of an
inclusion body of the target peptide efficiently.
free
free
free
