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10.1038/srep02203

http://scihub22266oqcxt.onion/10.1038/srep02203
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C3712317!3712317!23857555
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suck abstract from ncbi


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pmid23857555      Sci+Rep 2013 ; 3 (ä): ä
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  • Zn- and Mg- Containing Tricalcium Phosphates-Based Adjuvants for Cancer Immunotherapy #MMPMID23857555
  • Wang X; Li X; Onuma K; Sogo Y; Ohno T; Ito A
  • Sci Rep 2013[]; 3 (ä): ä PMID23857555show ga
  • Zn-, and Mg-containing tricalcium phosphates (TCPs) loaded with a hydrothermal extract of a human tubercle bacillus (HTB) were prepared by immersing Zn-TCP and Mg-TCP in HTB-containing supersaturated calcium phosphate solutions. The in vitro and in vivo immunogenic activities of the HTB-loaded Zn-, and Mg-TCPs (Zn-Ap-HTB and Mg-Ap-HTB, respectively) were evaluated as potential immunopotentiating adjuvants for cancer immunotherapy. The Zn-Ap-HTB and Mg-Ap-HTB adjuvants showed no obvious cytotoxicity and more effectively stimulated granulocyte-macrophage colony-stimulating factor (GM-CSF) secretion by macrophage-like cells than unprocessed HTB or HTB-loaded TCP (T-Ap-HTB) in vitro. Zn-Ap-HTB and Mg-Ap-HTB mixed with liquid-nitrogen-treated tumor tissue markedly inhibited the in vivo development of rechallenged Lewis lung carcinoma (LLC) cells compared with T-Ap-HTB and the unprocessed HTB mixed liquid-nitrogen-treated tumor tissue. Zn-Ap-HTB and Mg-Ap-HTB contributed to eliciting potent systemic antitumor immunity in vivo.
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