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10.1021/cn400012b http://scihub22266oqcxt.onion/10.1021/cn400012b C3689190!3689190 !23452507     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=23452507 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\23452507 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       ACS+Chem+Neurosci 2013 ; 4 (6 ): 963-72 Nephropedia Template TP {{tp|p=23452507 |t=2013. Improved orange and red Caȱ indicators and photophysical considerations for optogenetic applications |pdf=|usr=}}{{23452507 }} gab.com Text Improved orange and red Caȱ indicators and photophysical considerations for optogenetic applications JO: ACS Chem Neurosci http://www.kidney.de/pget.php?&tw=1&pmid=23452507 #FOAvip We have used protein engineering to expand the palette of genetically encoded calcium ion (Ca(2+)) indicators to include orange and improved red fluorescent variants, and validated the latter for combined use with optogenetic activation by channelrhodopsin-2 (ChR2). These indicators feature intensiometric signal changes that are 1.7- to 9.7-fold improved relatively to the progenitor Ca(2+) indicator, R-GECO1. In the course of this work, we discovered a photoactivation phenomenon in red fluorescent Ca(2+) indicators that, if not appreciated and accounted for, can cause false-positive artifacts in Ca(2+) imaging traces during optogenetic activation with ChR2. We demonstrate, in both a beta cell line and slice culture of developing mouse neocortex, that these artifacts can be avoided by using an appropriately low intensity of blue light for ChR2 activation. Twit Text FOAVip Improved orange and red Caȱ indicators and photophysical considerations for optogenetic applications ????ACS Chem Neurosci http://www.kidney.de/pget.php?&tw=1&pmid=23452507 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23452507 #FOAvip English Wikipedia <ref>{{Cite pmid|23452507 }}</ref> Improved orange and red Caȱ indicators and photophysical considerations for optogenetic applications #MMPMID23452507 Wu J ; Liu L ; Matsuda T ; Zhao Y ; Rebane A ; Drobizhev M ; Chang YF ; Araki S ; Arai Y ; March K ; Hughes TE ; Sagou K ; Miyata T ; Nagai T ; Li WH ; Campbell RE ACS Chem Neurosci 2013[Jun]; 4 (6 ): 963-72 PMID23452507 show ga We have used protein engineering to expand the palette of genetically encoded calcium ion (Ca(2+)) indicators to include orange and improved red fluorescent variants, and validated the latter for combined use with optogenetic activation by channelrhodopsin-2 (ChR2). These indicators feature intensiometric signal changes that are 1.7- to 9.7-fold improved relatively to the progenitor Ca(2+) indicator, R-GECO1. In the course of this work, we discovered a photoactivation phenomenon in red fluorescent Ca(2+) indicators that, if not appreciated and accounted for, can cause false-positive artifacts in Ca(2+) imaging traces during optogenetic activation with ChR2. We demonstrate, in both a beta cell line and slice culture of developing mouse neocortex, that these artifacts can be avoided by using an appropriately low intensity of blue light for ChR2 activation. |*Photochemical Processes [MESH] |Animals [MESH] |Calcium/*metabolism [MESH] |Cerebral Cortex/chemistry/metabolism [MESH] |Channelrhodopsins [MESH] |HEK293 Cells [MESH] |HeLa Cells [MESH] |Humans [MESH] |Indicators and Reagents/metabolism [MESH] |Mice [MESH] |Optogenetics/*methods [MESH] |Organ Culture Techniques [MESH] |Protein Engineering/*methods [MESH] |Protein Structure, Secondary [MESH]Improved orange and red Caȱ indicators and photophysical consideratio(epmc).pdf DeepDyve Pubget Overpricing