Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1152/ajprenal.00444.2012 http://scihub22266oqcxt.onion/10.1152/ajprenal.00444.2012 C3680677!3680677 !23552864     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=23552864 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\23552864 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Am+J+Physiol+Renal+Physiol 2013 ; 304 (12 ): F1428-37 Nephropedia Template TP {{tp|p=23552864 |t=2013. ?ENaC is required for whole cell mechanically gated currents in renal vascular smooth muscle cells |pdf=|usr=}}{{23552864 }} gab.com Text ENaC is required for whole cell mechanically gated currents in renal vascular smooth muscle cells JO: Am J Physiol Renal Physiol http://www.kidney.de/pget.php?&tw=1&pmid=23552864 #FOAvip Myogenic constrictor responses in small renal arteries and afferent arterioles are suppressed in mice with reduced levels of ?-epithelial Na? channel (?ENaC(m/m)). The underlying mechanism is unclear. Decreased activity of voltage-gated calcium channels (VGCC) or mechanically gated ion channels and increased activity of large conductance calcium-activated potassium (BK) channels are a few possible mechanisms. The purpose of this study was to determine if VGCC, BK, or mechanically gated ion channel activity was altered in renal vascular smooth muscle cell (VSMC) from ?ENaC(m/m) mice. To address this, we used whole cell patch-clamp electrophysiological approaches in freshly isolated renal VSMCs. Compared with ?ENaC(+/+) controls, the current-voltage relationships for VGCC and BK activity are similar in ?ENaC(m/m) mice. These findings suggest neither VGCC nor BK channel dysfunction accounts for reduced myogenic constriction in ?ENaC(m/m) mice. We then examined mechanically gated currents using a novel in vitro assay where VSMCs are mechanically activated by stretching an underlying elastomer. We found the mechanically gated currents, predominantly carried by Na?, are observed with less frequency (87 vs. 43%) and have smaller magnitude (-54.1 ± 12.5 vs. -20.9 ± 4.9 pA) in renal VSMCs from ?ENaC(m/m) mice. Residual currents are expected in this model since VSMC ?ENaC expression is reduced by 50%. These findings suggest ?ENaC is required for normal mechanically gated currents in renal VSMCs and their disruption may account for the reduced myogenic constriction in the ?ENaC(m/m) model. Our findings are consistent with the role of ?ENaC as a VSMC mechanosensor and function of evolutionarily related nematode degenerin proteins. Twit Text FOAVip ENaC is required for whole cell mechanically gated currents in renal vascular smooth muscle cells ????Am J Physiol Renal Physiol http://www.kidney.de/pget.php?&tw=1&pmid=23552864 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23552864 #FOAvip English Wikipedia <ref>{{Cite pmid|23552864 }}</ref> ?ENaC is required for whole cell mechanically gated currents in renal vascular smooth muscle cells #MMPMID23552864 Chung WS ; Weissman JL ; Farley J ; Drummond HA Am J Physiol Renal Physiol 2013[Jun]; 304 (12 ): F1428-37 PMID23552864 show ga Myogenic constrictor responses in small renal arteries and afferent arterioles are suppressed in mice with reduced levels of ?-epithelial Na? channel (?ENaC(m/m)). The underlying mechanism is unclear. Decreased activity of voltage-gated calcium channels (VGCC) or mechanically gated ion channels and increased activity of large conductance calcium-activated potassium (BK) channels are a few possible mechanisms. The purpose of this study was to determine if VGCC, BK, or mechanically gated ion channel activity was altered in renal vascular smooth muscle cell (VSMC) from ?ENaC(m/m) mice. To address this, we used whole cell patch-clamp electrophysiological approaches in freshly isolated renal VSMCs. Compared with ?ENaC(+/+) controls, the current-voltage relationships for VGCC and BK activity are similar in ?ENaC(m/m) mice. These findings suggest neither VGCC nor BK channel dysfunction accounts for reduced myogenic constriction in ?ENaC(m/m) mice. We then examined mechanically gated currents using a novel in vitro assay where VSMCs are mechanically activated by stretching an underlying elastomer. We found the mechanically gated currents, predominantly carried by Na?, are observed with less frequency (87 vs. 43%) and have smaller magnitude (-54.1 ± 12.5 vs. -20.9 ± 4.9 pA) in renal VSMCs from ?ENaC(m/m) mice. Residual currents are expected in this model since VSMC ?ENaC expression is reduced by 50%. These findings suggest ?ENaC is required for normal mechanically gated currents in renal VSMCs and their disruption may account for the reduced myogenic constriction in the ?ENaC(m/m) model. Our findings are consistent with the role of ?ENaC as a VSMC mechanosensor and function of evolutionarily related nematode degenerin proteins. |Animals [MESH] |Epithelial Sodium Channels/*physiology [MESH] |Ion Channel Gating/*physiology [MESH] |Large-Conductance Calcium-Activated Potassium Channels/physiology [MESH] |Mechanoreceptors/*physiology [MESH] |Mice [MESH] |Muscle, Smooth, Vascular/cytology/*physiology [MESH] |Patch-Clamp Techniques [MESH] |Potassium Channels, Voltage-Gated/physiology [MESH]?ENaC is required for whole cell mechanically gated currents in renal (epmc).pdf DeepDyve Pubget Overpricing