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10.1093/bioinformatics/bts596

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suck abstract from ncbi


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pmid23047559
      Bioinformatics 2013 ; 29 (12 ): 1485-7
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  • Promoter-proximal CCCTC-factor binding is associated with an increase in the transcriptional pausing index #MMPMID23047559
  • Paredes SH ; Melgar MF ; Sethupathy P
  • Bioinformatics 2013[Jun]; 29 (12 ): 1485-7 PMID23047559 show ga
  • MOTIVATION: It has been known for more than 2 decades that after RNA polymerase II (RNAPII) initiates transcription, it can enter into a paused or stalled state immediately downstream of the transcription start site before productive elongation. Recent advances in high-throughput genomic technologies facilitated the discovery that RNAPII pausing at promoters is a widespread physiologically regulated phenomenon. The molecular underpinnings of pausing are incompletely understood. The CCCTC-factor (CTCF) is a ubiquitous nuclear factor that has diverse regulatory functions, including a recently discovered role in promoting RNAPII pausing at splice sites. RESULTS: In this study, we analyzed CTCF binding sites and nascent transcriptomic data from three different cell types, and found that promoter-proximal CTCF binding is significantly associated with RNAPII pausing.
  • |*Promoter Regions, Genetic [MESH]
  • |*Transcription, Genetic [MESH]
  • |Animals [MESH]
  • |Binding Sites [MESH]
  • |CCCTC-Binding Factor [MESH]
  • |Cell Line [MESH]
  • |Cells, Cultured [MESH]
  • |Humans [MESH]
  • |Mice [MESH]
  • |RNA Polymerase II/*metabolism [MESH]


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