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2013 ; 139
(6
): 771-9
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gab.com Text
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Usefulness of a monoclonal ERG/FLI1 antibody for immunohistochemical
discrimination of Ewing family tumors
#MMPMID23690120
Tomlins SA
; Palanisamy N
; Brenner JC
; Stall JN
; Siddiqui J
; Thomas DG
; Lucas DR
; Chinnaiyan AM
; Kunju LP
Am J Clin Pathol
2013[Jun]; 139
(6
): 771-9
PMID23690120
show ga
Ewing family tumors (EFTs) and prostate carcinomas are characterized by
rearrangement of ETS genes, most commonly FLI1 (EFTs) and ERG (prostate
carcinomas). Previously, we characterized an antibody against ERG (EPR3864) for
detecting ERG-rearranged prostate carcinoma. Because EPR3864 also cross-reacts
with FLI1, we evaluated the usefulness of EPR3864 for discriminating EFTs from
other small round blue cell tumors (SRBCTs) with immunohistochemistry. Of 57
evaluable EFTs, 47 (82%) demonstrated at least moderate, diffuse, nuclear
ERG/FLI1 staining (including 89% and 100% of cases with confirmed EWSR1:FLI1 and
EWSR1:ERG fusions, respectively), of which 1, 3, and 43 showed negative,
cytoplasmic, or membranous CD99 staining, respectively. Among other SRBCTs (61
cases, 7 types), at least moderate, diffuse, nuclear EPR3864 staining was seen in
all precursor B-lymphoblastic lymphomas/leukemias and subsets of Burkitt
lymphomas (10%) and synovial sarcomas (45%). In summary, EPR3864 may be useful in
detecting EWSR1:FLI1 and EWSR1:ERG rearranged EFTs in addition to prostate
carcinomas.
|*Antibodies, Monoclonal
[MESH]
|12E7 Antigen
[MESH]
|Adolescent
[MESH]
|Adult
[MESH]
|Antigens, CD/analysis
[MESH]
|Calmodulin-Binding Proteins/*immunology
[MESH]
|Cell Adhesion Molecules/analysis
[MESH]
|Female
[MESH]
|Humans
[MESH]
|Immunohistochemistry
[MESH]
|Male
[MESH]
|Neoplasm Proteins
[MESH]
|Prostatic Neoplasms/diagnosis
[MESH]
|Proto-Oncogene Protein c-fli-1/*immunology
[MESH]