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10.1016/j.bbamem.2013.02.011 http://scihub22266oqcxt.onion/10.1016/j.bbamem.2013.02.011 C3625923!3625923!23454084     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biochim+Biophys+Acta 2013 ; 1828 (6): 1511-20 Nephropedia Template TP {{tp|p=23454084|t=2013. Real-time Attack of LL-37 on Single Bacillus subtilis Cells |pdf=|usr=}}{{23454084}} gab.com Text Real-time Attack of LL-37 on Single Bacillus subtilis Cells JO: Biochim Biophys Acta http://www.kidney.de/pget.php?&tw=1&pmid=23454084 #FOAvip Time-lapse fluorescence microscopy of single, growing Bacillus subtilis cells with 2-12 s time resolution reveals the mechanisms of antimicrobial peptide (AMP) action on a Gram-positive species with unprecedented detail. For the human cathelicidin LL-37 attacking B. subtilis, the symptoms of antimicrobial stress differ dramatically depending on the bulk AMP concentration. At 2 ?M LL-37, the mean single-cell growth rate decreases, but membrane permeabilization does not occur. At 4 ?M LL-37, cells abruptly shrink in size at the same time that Sytox Green enters the cytoplasm and stains the nucleoids. We interpret the shrinkage event as loss of turgor pressure (and presumably the membrane potential) due to permeabilization of the membrane. Movies of Sytox Green staining at 0.5 frame/s show that nucleoid staining is initially local, more consistent with pore formation than with global permeabilization models. In a novel ?growth recovery? assay, cells are incubated with LL-37 for a variable period and then rinsed with fresh growth medium lacking LL-37. The growth rate attenuation observed at 2 ?M LL-37 is a recoverable symptom, while the abrupt cell shrinkage observed at 4 ?M LL-37 is not. Twit Text FOAVip Real-time Attack of LL-37 on Single Bacillus subtilis Cells ????Biochim Biophys Acta http://www.kidney.de/pget.php?&tw=1&pmid=23454084 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23454084 #FOAvip English Wikipedia <ref>{{Cite pmid|23454084}}</ref> Real-time Attack of LL-37 on Single Bacillus subtilis Cells #MMPMID23454084 Barns KJ; Weisshaar JC Biochim Biophys Acta 2013[Jun]; 1828 (6): 1511-20 PMID23454084show ga Time-lapse fluorescence microscopy of single, growing Bacillus subtilis cells with 2-12 s time resolution reveals the mechanisms of antimicrobial peptide (AMP) action on a Gram-positive species with unprecedented detail. For the human cathelicidin LL-37 attacking B. subtilis, the symptoms of antimicrobial stress differ dramatically depending on the bulk AMP concentration. At 2 ?M LL-37, the mean single-cell growth rate decreases, but membrane permeabilization does not occur. At 4 ?M LL-37, cells abruptly shrink in size at the same time that Sytox Green enters the cytoplasm and stains the nucleoids. We interpret the shrinkage event as loss of turgor pressure (and presumably the membrane potential) due to permeabilization of the membrane. Movies of Sytox Green staining at 0.5 frame/s show that nucleoid staining is initially local, more consistent with pore formation than with global permeabilization models. In a novel ?growth recovery? assay, cells are incubated with LL-37 for a variable period and then rinsed with fresh growth medium lacking LL-37. The growth rate attenuation observed at 2 ?M LL-37 is a recoverable symptom, while the abrupt cell shrinkage observed at 4 ?M LL-37 is not. äReal-time Attack of LL-37 on Single Bacillus subtilis Cells (epmc).pdf DeepDyve Pubget Overpricing