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2012 ; 136
(4
): 641-8
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Resolution of immune response by recombinant transforming growth factor-beta
(rTGF-?) during influenza A virus infection
#MMPMID23168705
Srivastava V
; Khanna M
; Sharma S
; Kumar B
Indian J Med Res
2012[Oct]; 136
(4
): 641-8
PMID23168705
show ga
BACKGROUND & OBJECTIVES: Replication of influenza A virus in the respiratory
tract leads to cell damage and liberation of cytokines and chemokines. The in
vivo cytokine induction and modulation by recombinant transforming growth factor-
?1 (rTGF-?1) has not been studied. Therefore, in the present study the effect of
rTGF-?1, a potent immunomodulatory cytokine which has anti-inflammatory
properties and downregulates the release of inflammatory molecules, against
influenza-virus infection in the airway of mice was investigated. METHODS:
rTGF-?1 was administered intravenously to mice with concomitant intranasal
infection of influenza A/Udorn/317/72 (H3N2) virus, and the survival rate, virus
titre, histopathological changes and levels of factors regulating inflammation in
the airway fluid were analysed. RESULT: The immune response to influenza A virus
was characterized by an influx of both macrophages and lymphocytes into the lungs
of the infected host. rTGF-?1 significantly suppressed virus multiplication and
improved the survival rate of mice. rTGF-?1 downregulated infiltration of
neutrophils and the release of inflammatory molecules, such as interferon-gamma
(IFN-?), interleukin-1 ? (IL-1?) and stimulated release of IL-10 that potentiates
anti-inflammatory response into airway. INTERPRETATION & CONCLUSIONS: A
generalized pulmonary inflammation does not contribute to viral clearance but
represents an immunological background within which antiviral immunity operates.
Treatment with rTGF-?1 reduced macrophage count and neutrophils influx in lungs
of infected mice.