Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=22895087
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Nonpathogenic Lactobacillus rhamnosus activates the inflammasome and antiviral
responses in human macrophages
#MMPMID22895087
Miettinen M
; Pietilä TE
; Kekkonen RA
; Kankainen M
; Latvala S
; Pirhonen J
; Österlund P
; Korpela R
; Julkunen I
Gut Microbes
2012[Nov]; 3
(6
): 510-22
PMID22895087
show ga
In this study, we have utilized global gene expression profiling to compare the
responses of human primary macrophages to two closely related, well-characterized
Lactobacillus rhamnosus strains GG and LC705, since our understanding of the
responses elicited by nonpathogenic bacteria in human innate immune system is
limited. Macrophages are phagocytic cells of the innate immune system that
perform sentinel functions to initiate appropriate responses to surrounding
stimuli. Macrophages that reside on gut mucosa encounter ingested and intestinal
bacteria. Bacteria of Lactobacillus genus are nonpathogenic and used in food and
as supplements with health-promoting probiotic potential. Our results demonstrate
that live GG and LC705 induced quantitatively different gene expression profiles
in macrophages. A gene ontology analysis revealed functional similarities and
differences in responses to GG and LC705 that were reflected in host defense
responses. Both GG and LC705 induced interleukin-1? production in macrophages
that required caspase-1 activity. LC705, but not GG, induced type I interferon
-dependent gene activation that correlated with its ability to prevent influenza
A virus replication and production of viral proteins in macrophages. Our results
indicate that nonpathogenic bacteria are able to activate the inflammasome. In
addition, our results suggest that L. rhamnosus may prime the antiviral potential
of human macrophages.
free
free
free
