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10.1073/pnas.1206039109 http://scihub22266oqcxt.onion/10.1073/pnas.1206039109 C3409765!3409765 !22778433     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\22778433 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Proc+Natl+Acad+Sci+U+S+A 2012 ; 109 (30 ): 12117-22 Nephropedia Template TP {{tp|p=22778433 |t=2012. Hematopoietic-specific targeting of influenza A virus reveals replication requirements for induction of antiviral immune responses |pdf=|usr=}}{{22778433 }} gab.com Text Hematopoietic-specific targeting of influenza A virus reveals replication requirements for induction of antiviral immune responses JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=22778433 #FOAvip A coordinated innate and adaptive immune response, orchestrated by antigen presenting cells (APCs), is required for effective clearance of influenza A virus (IAV). Although IAV primarily infects epithelial cells of the upper respiratory tract, APCs are also susceptible. To determine if virus transcription in these cells is required to generate protective innate and adaptive immune responses, we engineered IAV to be selectively attenuated in cells of hematopoietic origin. Incorporation of hematopoietic-specific miR-142 target sites into the nucleoprotein of IAV effectively silenced virus transcription in APCs, but had no significant impact in lung epithelial cells. Here we demonstrate that inhibiting IAV replication in APCs in vivo did not alter clearance, or the generation of IAV-specific CD8 T cells, suggesting that cross-presentation is sufficient for cytotoxic T lymphocyte activation. In contrast, loss of in vivo virus infection, selectively in APCs, resulted in a significant reduction of retinoic acid-inducible gene I-dependent type I IFN (IFN-I). These data implicate the formation of virus replication intermediates in APCs as the predominant trigger of IFN-I in vivo. Taking these data together, this research describes a unique platform to study the host response to IAV and provides insights into the mechanism of antigen presentation and the induction of IFN-I. Twit Text FOAVip Hematopoietic-specific targeting of influenza A virus reveals replication requirements for induction of antiviral immune responses ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=22778433 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22778433 #FOAvip English Wikipedia <ref>{{Cite pmid|22778433 }}</ref> Hematopoietic-specific targeting of influenza A virus reveals replication requirements for induction of antiviral immune responses #MMPMID22778433 Langlois RA ; Varble A ; Chua MA ; García-Sastre A ; tenOever BR Proc Natl Acad Sci U S A 2012[Jul]; 109 (30 ): 12117-22 PMID22778433 show ga A coordinated innate and adaptive immune response, orchestrated by antigen presenting cells (APCs), is required for effective clearance of influenza A virus (IAV). Although IAV primarily infects epithelial cells of the upper respiratory tract, APCs are also susceptible. To determine if virus transcription in these cells is required to generate protective innate and adaptive immune responses, we engineered IAV to be selectively attenuated in cells of hematopoietic origin. Incorporation of hematopoietic-specific miR-142 target sites into the nucleoprotein of IAV effectively silenced virus transcription in APCs, but had no significant impact in lung epithelial cells. Here we demonstrate that inhibiting IAV replication in APCs in vivo did not alter clearance, or the generation of IAV-specific CD8 T cells, suggesting that cross-presentation is sufficient for cytotoxic T lymphocyte activation. In contrast, loss of in vivo virus infection, selectively in APCs, resulted in a significant reduction of retinoic acid-inducible gene I-dependent type I IFN (IFN-I). These data implicate the formation of virus replication intermediates in APCs as the predominant trigger of IFN-I in vivo. Taking these data together, this research describes a unique platform to study the host response to IAV and provides insights into the mechanism of antigen presentation and the induction of IFN-I. |Adaptive Immunity/*immunology [MESH] |Animals [MESH] |Antigen-Presenting Cells/*virology [MESH] |Base Sequence [MESH] |Blotting, Northern [MESH] |Blotting, Western [MESH] |Cell Line [MESH] |Cloning, Molecular [MESH] |DNA Primers/genetics [MESH] |Dogs [MESH] |Fibroblasts [MESH] |Flow Cytometry [MESH] |Gene Expression Regulation, Viral/genetics/*immunology [MESH] |High-Throughput Nucleotide Sequencing [MESH] |Humans [MESH] |Immunity, Innate/*immunology [MESH] |Influenza A virus/*immunology [MESH] |Interferon Type I/metabolism [MESH] |Macrophages [MESH] |Mice [MESH] |MicroRNAs/metabolism [MESH] |Molecular Sequence Data [MESH] |Nucleoproteins/genetics/metabolism [MESH]Hematopoietic-specific targeting of influenza A virus reveals replicat(epmc).pdf DeepDyve Pubget Overpricing