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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Stem+Cells+Dev
2010 ; 19
(9
): 1393-403
Nephropedia Template TP
gab.com Text
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Twit Text #
English Wikipedia
Transient receptor potential melastatin type 7 channel is critical for the
survival of bone marrow derived mesenchymal stem cells
#MMPMID19929312
Cheng H
; Feng JM
; Figueiredo ML
; Zhang H
; Nelson PL
; Marigo V
; Beck A
Stem Cells Dev
2010[Sep]; 19
(9
): 1393-403
PMID19929312
show ga
The transient receptor potential melastatin type 7 channel (TRPM7) is a member of
the TRP family of ion channels that is essential for cell proliferation and
viability. Mesenchymal stem cells (MSCs) from bone marrow are a potential source
for tissue repair due to their ability to differentiate into specialized cells.
However, the role of TRPM7 in stem cells is unknown. In this study, we
characterized TRPM7 in mouse MSCs using molecular biology, immunocytochemistry,
and patch clamp. We also investigated TRPM7 function using a lentiviral vector
and specific shRNA to knockdown gene expression. By RT-PCR and
immunocytochemistry, we identified TRPM7, but not TRPM6, a close family member
with similar function. Electrophysiological recordings during depletion of
intracellular Mg(2+) or Mg(2+)-ATP resulted in the development of currents
typical for the channel. Furthermore, 2-aminoethoxydiphenyl borate (1 pM-100
microM) inhibited TRPM7 in a concentration-dependent manner. The molecular
suppression of TRPM7 significantly decreased MSC proliferation and viability as
determined by MTT assay. In addition, TRPM7 gene expression was up-regulated
during osteogenesis. These findings demonstrate that TRPM7 is required for MSC
survival and perhaps involved in the differentiation process.
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