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10.1152/jn.00759.2010 http://scihub22266oqcxt.onion/10.1152/jn.00759.2010 C3118738!3118738!21430274     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Neurophysiol 2011 ; 105 (6): 2791-801 Nephropedia Template TP {{tp|p=21430274|t=2011. Involvement of TRP channels in the CO2 chemosensitivity of locus coeruleus neurons |pdf=|usr=}}{{21430274}} gab.com Text Involvement of TRP channels in the CO2 chemosensitivity of locus coeruleus neurons JO: J Neurophysiol http://www.kidney.de/pget.php?&tw=1&pmid=21430274 #FOAvip Catecholaminergic neurons in the locus coeruleus (LC) play a role in the ventilatory response to hypercapnia. Here, we show evidence for the involvement of transient receptor potential (TRP) channels. We found that the input resistance was reduced during an exposure to 8% CO2 in ?35% LC neurons in mouse brain slices, accompanied by depolarization and higher firing activity. The neuronal responses suggest the opening of Na+ or nonselective cationic channels instead of the closure of K+ channels. As a major group of cationic channels, the TRP channels are expressed in the brain, some of which are activated by acidic pH. We therefore screened all representative TRP channels using the quantitative real-time PCR analysis. High levels of mRNA expression of TRPC5, TRPM2, and TRPM7 were found in the LC tissue. Of them, the TRPC5 transcript was the most abundant. The TRPC5 channel was activated by extracellular acidification when expressed in human embryonic kidney (HEK) cells. The TRPC5 currents started to be activated at pH 7.4 with pKa 6.9. The TRPC5 currents were also activated by isohydric hypercapnic and intracellular acidosis in a Ca2+-dependent manner. Consistently, the LC neurons were stimulated by both extra- and intracellular acidosis. The stimulatory effect of hypercapnia on LC neurons was eliminated by selective TRPC inhibitor SKF-96365 with and without the blockade of synaptic transmission. Single-cell PCR analysis indicated that TRPC5 mRNAs existed in the LC neurons. Thus these results strongly suggest that the TRP channels are likely to play a role in the CO2 chemosensitivity of LC neurons, especially TRPC5. Twit Text FOAVip Involvement of TRP channels in the CO2 chemosensitivity of locus coeruleus neurons ????J Neurophysiol http://www.kidney.de/pget.php?&tw=1&pmid=21430274 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=21430274 #FOAvip English Wikipedia <ref>{{Cite pmid|21430274}}</ref> Involvement of TRP channels in the CO2 chemosensitivity of locus coeruleus neurons #MMPMID21430274 Cui N; Zhang X; Tadepalli JS; Yu L; Gai H; Petit J; Pamulapati RT; Jin X; Jiang C J Neurophysiol 2011[Jun]; 105 (6): 2791-801 PMID21430274show ga Catecholaminergic neurons in the locus coeruleus (LC) play a role in the ventilatory response to hypercapnia. Here, we show evidence for the involvement of transient receptor potential (TRP) channels. We found that the input resistance was reduced during an exposure to 8% CO2 in ?35% LC neurons in mouse brain slices, accompanied by depolarization and higher firing activity. The neuronal responses suggest the opening of Na+ or nonselective cationic channels instead of the closure of K+ channels. As a major group of cationic channels, the TRP channels are expressed in the brain, some of which are activated by acidic pH. We therefore screened all representative TRP channels using the quantitative real-time PCR analysis. High levels of mRNA expression of TRPC5, TRPM2, and TRPM7 were found in the LC tissue. Of them, the TRPC5 transcript was the most abundant. The TRPC5 channel was activated by extracellular acidification when expressed in human embryonic kidney (HEK) cells. The TRPC5 currents started to be activated at pH 7.4 with pKa 6.9. The TRPC5 currents were also activated by isohydric hypercapnic and intracellular acidosis in a Ca2+-dependent manner. Consistently, the LC neurons were stimulated by both extra- and intracellular acidosis. The stimulatory effect of hypercapnia on LC neurons was eliminated by selective TRPC inhibitor SKF-96365 with and without the blockade of synaptic transmission. Single-cell PCR analysis indicated that TRPC5 mRNAs existed in the LC neurons. Thus these results strongly suggest that the TRP channels are likely to play a role in the CO2 chemosensitivity of LC neurons, especially TRPC5. äInvolvement of TRP channels in the CO2 chemosensitivity of locus coeru(epmc).pdf DeepDyve Pubget Overpricing