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10.1681/ASN.2009111153 http://scihub22266oqcxt.onion/10.1681/ASN.2009111153 C2938592!2938592!20595681     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Am+Soc+Nephrol 2010 ; 21 (8): 1309-16 Nephropedia Template TP {{tp|p=20595681|t=2010. Effects of the EGFR Inhibitor Erlotinib on Magnesium Handling |pdf=|usr=}}{{20595681}} gab.com Text Effects of the EGFR Inhibitor Erlotinib on Magnesium Handling JO: J Am Soc Nephrol http://www.kidney.de/pget.php?&tw=1&pmid=20595681 #FOAvip A mutation in pro-EGF causes isolated hypomagnesemia, and monoclonal antibodies targeting the extracellular domain of the EGF receptor (EGFR) affect epithelial Mg2+ transport. The effect of the EGFR tyrosine kinase inhibitor erlotinib on Mg2+ homeostasis, however, remains unknown. Here, we injected C57BL/6 mice with erlotinib for 23 days and observed a small but significant decrease in serum Mg2+ concentrations at days 16 and 23, but the fractional excretion of Mg2+ remained unchanged after 23 days. Semiquantitative immunohistochemical evaluation did not reveal detectable changes in renal expression of transient receptor potential melastatin 6 (TRPM6) protein, the channel that mediates Mg2+ reabsorption. Patch clamp analysis in TRPM6-expressing cells demonstrated that 30 ?M erlotinib inhibited EGF-induced changes in TRPM6 current density and tyrosine phosphorylation of EGFR; 0.3 ?M erlotinib did not have these effects. Furthermore, 30 ?M erlotinib inhibited EGF-stimulated increases in the mobile fraction of endomembrane TRPM6 channels. In summary, erlotinib can influence Mg2+ handling but its effect on the systemic Mg2+ concentration seems less potent than that observed with antibody-based EGFR inhibitors. These data suggest that typical human dosages of erlotinib are unlikely to severely affect serum Mg2+ concentrations. Twit Text FOAVip Effects of the EGFR Inhibitor Erlotinib on Magnesium Handling ????J Am Soc Nephrol http://www.kidney.de/pget.php?&tw=1&pmid=20595681 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=20595681 #FOAvip English Wikipedia <ref>{{Cite pmid|20595681}}</ref> Effects of the EGFR Inhibitor Erlotinib on Magnesium Handling #MMPMID20595681 Dimke H; van der Wijst J; Alexander TR; Meijer IM; Mulder GM; van Goor H; Tejpar S; Hoenderop JG; Bindels RJ J Am Soc Nephrol 2010[Aug]; 21 (8): 1309-16 PMID20595681show ga A mutation in pro-EGF causes isolated hypomagnesemia, and monoclonal antibodies targeting the extracellular domain of the EGF receptor (EGFR) affect epithelial Mg2+ transport. The effect of the EGFR tyrosine kinase inhibitor erlotinib on Mg2+ homeostasis, however, remains unknown. Here, we injected C57BL/6 mice with erlotinib for 23 days and observed a small but significant decrease in serum Mg2+ concentrations at days 16 and 23, but the fractional excretion of Mg2+ remained unchanged after 23 days. Semiquantitative immunohistochemical evaluation did not reveal detectable changes in renal expression of transient receptor potential melastatin 6 (TRPM6) protein, the channel that mediates Mg2+ reabsorption. Patch clamp analysis in TRPM6-expressing cells demonstrated that 30 ?M erlotinib inhibited EGF-induced changes in TRPM6 current density and tyrosine phosphorylation of EGFR; 0.3 ?M erlotinib did not have these effects. Furthermore, 30 ?M erlotinib inhibited EGF-stimulated increases in the mobile fraction of endomembrane TRPM6 channels. In summary, erlotinib can influence Mg2+ handling but its effect on the systemic Mg2+ concentration seems less potent than that observed with antibody-based EGFR inhibitors. These data suggest that typical human dosages of erlotinib are unlikely to severely affect serum Mg2+ concentrations. äEffects of the EGFR Inhibitor Erlotinib on Magnesium Handling (epmc).pdf DeepDyve Pubget Overpricing