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2003 ; 84
(3
): 127-33
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Effect of Quercetin on lipid peroxidation and changes in lung morphology in
experimental influenza virus infection
#MMPMID12974942
Kumar P
; Sharma S
; Khanna M
; Raj HG
Int J Exp Pathol
2003[Jun]; 84
(3
): 127-33
PMID12974942
show ga
Influenza virus infection, induced experimentally in mice, was associated with
marked changes in lung morphology viz. epithelial damage with focal areas of
reactive papillary hyperplasia, infiltration of leukocytes and development of
oxidative stress, as evidenced by increased superoxide radical production and
lipid peroxidation (LPO) products by alveolar macrophages. These effects were
observed on the 5th day after virus instillation. The levels of superoxide and
LPO were measured spectrophotometrically by the nitroblue tetrazolium (NBT) assay
and thiobarbituric acid reactive species (TBARS) assay, respectively. The former
increased by 1.5-2 fold and the latter was raised by 85% when compared with
normal control. Supplementation of intranasal viral instillation with the
anti-oxidant, Quercetin, given orally, resulted in a significant decrease in the
levels of both superoxide radicals and LPO products. There was also a significant
decrease in the number of infiltrating cells. A mild to moderate protective
effect was observed in lung morphology. Thus, Quercetin may be useful as a drug
in reducing the oxidative stress induced by influenza virus infection in the
lung, and protect it from the toxic effects of the free radicals.