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10.1073/pnas.0800881105 http://scihub22266oqcxt.onion/10.1073/pnas.0800881105 C2422775!2422775!18539771     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2008 ; 105 (24): 8304-8 Nephropedia Template TP {{tp|p=18539771|t=2008. TRPM7 facilitates cholinergic vesicle fusion with the plasma membrane |pdf=|usr=}}{{18539771}} gab.com Text TRPM7 facilitates cholinergic vesicle fusion with the plasma membrane JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=18539771 #FOAvip TRPM7, of the transient receptor potential (TRP) family, is both an ion channel and a kinase. Previously, we showed that TRPM7 is located in the membranes of acetylcholine (ACh)-secreting synaptic vesicles of sympathetic neurons, forms a molecular complex with proteins of the vesicular fusion machinery, and is critical for stimulated neurotransmitter release. Here, we targeted pHluorin to small synaptic-like vesicles (SSLV) in PC12 cells and demonstrate that it can serve as a single-vesicle plasma membrane fusion reporter. In PC12 cells, as in sympathetic neurons, TRPM7 is located in ACh-secreting SSLVs. TRPM7 knockdown by siRNA, or abolishing channel activity by expression of a dominant negative TRPM7 pore mutant, decreased the frequency of spontaneous and voltage-stimulated SSLV fusion events without affecting large dense core vesicle secretion. We conclude that the conductance of TRPM7 across the vesicle membrane is important in SSLV fusion. Twit Text FOAVip TRPM7 facilitates cholinergic vesicle fusion with the plasma membrane ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=18539771 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=18539771 #FOAvip English Wikipedia <ref>{{Cite pmid|18539771}}</ref> TRPM7 facilitates cholinergic vesicle fusion with the plasma membrane #MMPMID18539771 Brauchi S; Krapivinsky G; Krapivinsky L; Clapham DE Proc Natl Acad Sci U S A 2008[Jun]; 105 (24): 8304-8 PMID18539771show ga TRPM7, of the transient receptor potential (TRP) family, is both an ion channel and a kinase. Previously, we showed that TRPM7 is located in the membranes of acetylcholine (ACh)-secreting synaptic vesicles of sympathetic neurons, forms a molecular complex with proteins of the vesicular fusion machinery, and is critical for stimulated neurotransmitter release. Here, we targeted pHluorin to small synaptic-like vesicles (SSLV) in PC12 cells and demonstrate that it can serve as a single-vesicle plasma membrane fusion reporter. In PC12 cells, as in sympathetic neurons, TRPM7 is located in ACh-secreting SSLVs. TRPM7 knockdown by siRNA, or abolishing channel activity by expression of a dominant negative TRPM7 pore mutant, decreased the frequency of spontaneous and voltage-stimulated SSLV fusion events without affecting large dense core vesicle secretion. We conclude that the conductance of TRPM7 across the vesicle membrane is important in SSLV fusion. äTRPM7 facilitates cholinergic vesicle fusion with the plasma membrane (epmc).pdf DeepDyve Pubget Overpricing