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C228048!228048!7790444
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suck abstract from ncbi


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pmid7790444      J+Clin+Microbiol 1995 ; 33 (4): 821-3
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  • Detection of Borna disease virus RNA in formalin-fixed, paraffin-embedded brain tissues by nested PCR #MMPMID7790444
  • Sorg I; Metzler A
  • J Clin Microbiol 1995[Apr]; 33 (4): 821-3 PMID7790444show ga
  • A method for detecting Borna disease virus (BDV) RNA in formalin-fixed, paraffin-embedded brain tissue sections was established. By digestion with proteinase K and subsequent extraction with guanidinium thiocyanate, phenol, and chloroform, we were able to efficiently release RNA from the fixed tissues. By reverse transcription of the RNA and nested PCR a 212-bp product was generated, as expected.
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