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1955 ; 101
(6
): 665-76
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Studies of influenza infection in ferrets by means of fluorescein-labelled
antibody I The pathogenesis and diagnosis of the disease
#MMPMID14367687
LIU C
J Exp Med
1955[Jun]; 101
(6
): 665-76
PMID14367687
show ga
By means of fluorescein-labelled antibody staining, specific influenza viral
antigens were seen in both the cytoplasm and the nucleus of infected ciliated
epithelial cells covering the nasal turbinates of infected ferrets. Initially,
only a small portion of the nasal epithelium showed fluorescence, and no
appreciable abnormality of the cells could be detected by hematoxylin and eosin
staining. The fluorescence soon spread to involve the entire epithelium, followed
by desquamation coinciding with the onset of manifest illness. Pneumonia was seen
in some of the infected ferrets, and in them, viral antigens were found in the
bronchial epithelium and in the mediastinal lymph nodes. A rise of viral
infectivity titer paralleled the observed spread of viral antigens. Many
desquamated nasal epithelial cells and macrophages containing antigen were
present in nasal smears. The finding would seem to offer a method for the rapid
specific diagnosis of influenza infection.