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10.1083/jcb.106.3.649 http://scihub22266oqcxt.onion/10.1083/jcb.106.3.649 C2115096!2115096 !2831233     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=2831233 &cmd=llinks): Failed to open stream: HTTP request failed! 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Murine fetal liver macrophages bind developing erythroblasts by a divalent cation-dependent hemagglutinin |pdf=|usr=}}{{2831233 }} gab.com Text Murine fetal liver macrophages bind developing erythroblasts by a divalent cation-dependent hemagglutinin JO: J Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=2831233 #FOAvip During mammalian development the fetal liver plays an important role in hematopoiesis. Studies with the macrophage (M phi)-specific mAb F4/80 have revealed an extensive network of M phi plasma membranes interspersed between developing erythroid cells in fetal liver. To investigate the interactions between erythroid cells and stromal M phi, we isolated hematopoietic cell clusters from embryonic day-14 murine fetal liver by collagenase digestion and adherence. Clusters of erythroid cells adhered to glass mainly via M phi, 94% of which bound 19 +/- 11 erythroblasts (Eb) per cell. Bound Eb proliferated vigorously on the surface of fetal liver M phi, with little evidence of ingestion. The M phi could be stripped of their associated Eb and the clusters then reconstituted by incubation with Eb in the presence of divalent cations. The interaction required less Ca++ than Mg++, 100 vs. 250 microM for half-maximal binding, and was mediated by a trypsin-sensitive hemagglutinin on the M phi surface. After trypsin treatment fetal liver M phi recovered the ability to bind Eb and this process could be selectively inhibited by cycloheximide. Inhibition tests showed that the Eb receptor differs from known M phi plasma membrane receptors and fetal liver M phi did not bind sheep erythrocytes, a ligand for a distinct M phi hemagglutinin. We propose that fetal liver M phi interact with developing erythroid cells by a novel nonphagocytic surface hemagglutinin which is specific for a ligand found on Eb and not on mature red cells. Twit Text FOAVip Murine fetal liver macrophages bind developing erythroblasts by a divalent cation-dependent hemagglutinin ????J Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=2831233 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=2831233 #FOAvip English Wikipedia <ref>{{Cite pmid|2831233 }}</ref> Murine fetal liver macrophages bind developing erythroblasts by a divalent cation-dependent hemagglutinin #MMPMID2831233 Morris L ; Crocker PR ; Gordon S J Cell Biol 1988[Mar]; 106 (3 ): 649-56 PMID2831233 show ga During mammalian development the fetal liver plays an important role in hematopoiesis. Studies with the macrophage (M phi)-specific mAb F4/80 have revealed an extensive network of M phi plasma membranes interspersed between developing erythroid cells in fetal liver. To investigate the interactions between erythroid cells and stromal M phi, we isolated hematopoietic cell clusters from embryonic day-14 murine fetal liver by collagenase digestion and adherence. Clusters of erythroid cells adhered to glass mainly via M phi, 94% of which bound 19 +/- 11 erythroblasts (Eb) per cell. Bound Eb proliferated vigorously on the surface of fetal liver M phi, with little evidence of ingestion. The M phi could be stripped of their associated Eb and the clusters then reconstituted by incubation with Eb in the presence of divalent cations. The interaction required less Ca++ than Mg++, 100 vs. 250 microM for half-maximal binding, and was mediated by a trypsin-sensitive hemagglutinin on the M phi surface. After trypsin treatment fetal liver M phi recovered the ability to bind Eb and this process could be selectively inhibited by cycloheximide. Inhibition tests showed that the Eb receptor differs from known M phi plasma membrane receptors and fetal liver M phi did not bind sheep erythrocytes, a ligand for a distinct M phi hemagglutinin. We propose that fetal liver M phi interact with developing erythroid cells by a novel nonphagocytic surface hemagglutinin which is specific for a ligand found on Eb and not on mature red cells. |*Hematopoiesis [MESH] |Animals [MESH] |Calcium/metabolism [MESH] |Cell Adhesion [MESH] |Cells, Cultured [MESH] |Cycloheximide/pharmacology [MESH] |Erythroblasts/*metabolism/ultrastructure [MESH] |Fetus [MESH] |Hemagglutinins/*metabolism [MESH] |Ligands [MESH] |Liver/*cytology/embryology [MESH] |Macrophages/drug effects/*metabolism/ultrastructure [MESH] |Magnesium/metabolism [MESH] |Mice [MESH] |Microbial Collagenase [MESH] |Microscopy, Electron, Scanning [MESH]Murine fetal liver macrophages bind developing erythroblasts by a diva(epmc).pdf DeepDyve Pubget Overpricing