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1981 ; 44
(1
): 17-28
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gab.com Text
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The inductive requirements for the primary in vitro generation of delayed-type
hypersensitivity response to influenza virus in mice
#MMPMID6792057
Leung KN
; Mak NK
; Ada GL
Immunology
1981[Sep]; 44
(1
): 17-28
PMID6792057
show ga
Effector T cells (Td) which mediate delayed-type hypersensitivity reactions to
influenza A virus can be generated in tissue culture using normal mouse spleen
cells as the responder population. Addition of helper T cells enhances but is not
essential for the production of Td cells. Both Ly 1 positive, I region restricted
and Ly 2,3 positive, K,D region restricted effector cells are generated. Treating
the responder cell population with anti-Ly 1 or anti-Ly 2,3 antibodies and
complement prevented the generation of both classes of effector T cell,
suggesting that the precursor Td cells are Ly 1,2,3 positive. Effector cells
which are specific for the homologous virus or cross-reactive within the A
strains of influenza virus are produced, as has been found previously in in vivo
experiments. Depleting the cell population of phagocytic and plastic adherent
cells resulted in a failure to produce Td cells, which showed a requirement for
macrophage-like cells as accessory cells in the primary in vitro generation of Td
cells. A variety of cells, such as peritoneal exudate cells, mitogen stimulated
blasts or L929 fibroblast cells could serve as stimulator cells. Only Ly 2,3
positive, K,D region-restricted Td cells were produced when L929 cells were used
as they lack I region-coded surface antigens. The I region-restricted DTH
response was mapped to the IA sub-region of the H-2 gene complex.