Various Bacillus and Paenibacillus Spp Isolated From Soil Produce Compounds With
Potent Antimicrobial Activity Against Clinically Relevant Pathogens
#MMPMID41381995
Moran M
; Turner H
; Yanchar J
; Preece J
; Ahlborn G
; Robison R
Microbiologyopen
2025[Dec]; 14
(6
): e70179
PMID41381995
show ga
The increasing prevalence of antibiotic resistance among clinically significant
pathogens necessitates the discovery of novel antimicrobial agents. This study
investigated 29 Bacillus and Paenibacillus isolates from the soil for
antimicrobial activity against multidrug-resistant clinical pathogens, including
methicillin-resistant Staphylococcus aureus (MRSA) and carbapenem-resistant
Enterobacterales (CRE). In both agar- and broth-based antimicrobial assays,
Paenibacillus profundus strains 7.5 and M4.5 exhibited potent broad-spectrum
activity, including significant inhibition of many CREs. Species identification
was performed through 16S rRNA sequencing, and genome mining of three producer
strains using antiSMASH revealed biosynthetic gene clusters associated with a
variety of nonribosomal peptide synthetases (NRPSs), polyketide synthases (PKSs),
and ribosomally synthesized and post-translationally modified peptides (RiPPs).
While many of these clusters were not associated with known antimicrobial
compounds, several of them displayed high similarity to known compounds such as
polymyxin B, paenilan, colistin, and paenibacterin. These findings reinforce
numerous previous studies highlighting the potential of soil-derived Bacillus and
Paenibacillus species as valuable sources of novel antimicrobials to address the
global antibiotic resistance crisis.
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