Computed tomography image analysis of cobalt oxide nanoparticles-induced
cytopathic effect on Hep-G2 cell lines
#MMPMID41384061
Nasr Eldin MS
; Soula MA
; Emara AA
; Ahmed HM
; Al-Bishri WM
; El-Sayyad GS
RSC Adv
2025[Dec]; 15
(57
): 49144-49158
PMID41384061
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The main aim of the present study is to examine the effect of cobalt oxide
nanoparticles (Co(3)O(4) NPs) on the proliferation of hepatocellular carcinoma
(Hep-G2) cells. The cells were exposed to red light with a wavelength of ? = 655
nm for 30 minutes at a power of 50 mW cm(-2) and in the dark. The sol-gel method
was used to create Co(3)O(4) NPs, which were then calcined at 800 °C. HRTEM, XRD,
SEM, FTIR, DLS, and zeta potential tests were used to examine Co(3)O(4) NPs and
to determine their shape, size, crystal structure, surface appearance and charge,
and size range. The results revealed that light-induced photodynamic treatment is
highly detrimental. MATLAB-R2010a and Fiji (ImageJ) open-source program powered
by the National Institutes of Health (US) software were used to analyze the
collected data to determine its quantitative or qualitative qualities through the
use of gray-level histogram analysis. After the tested cells were looked at in
grayscale images made with computed tomography (CT) and Co(3)O(4) NPs, we found
that there was a broad peak in the middle. On the other hand, the histogram shows
a leftward skew and shift with a peak that has a wide base and a sharp top or
bottom in cell lines. This study provides strong evidence that Co(3)O(4) NPs can
stop Hep-G2 cell lines from multiplying. The data from CT images of Co(3)O(4) NPs
showed a high degree of statistical significance. This work summarizes the use of
metallic NPs, particularly Co(3)O(4) NPs, in CT imaging to detect and treat
cancer.
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