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The molecular mechanism of leaf margin fission in Solanum nigrum revealed by
combined PPI network and WGCNA and functional validation of SnNAC90
#MMPMID41346828
Shen H
; Miao M
; Cha Z
; Fan Z
; Cai Q
; Sun Z
; Li L
Front Plant Sci
2025[]; 16
(?): 1706416
PMID41346828
show ga
INTRODUCTION: Solanum nigrum, a member of the Solanaceae family, holds
significant importance in various aspects, including edible and medicinal uses,
ecological management, and environmental landscaping. The leaf edges of S. nigrum
exhibit 4-5 significant serrations, and the splitting of these leaf margins plays
a crucial role in the plant's adaptation to environmental shifts. METHODS: In
this study, we systematically analyzed the molecular mechanism of leaf margin
fission in S. nigrum by combining RNA sequencing, weighted gene co-expression
network analysis, and overexpression transgenic technology using leaves from five
growth stages of S. nigrum during the flowering period as materials. RESULTS:
Transcriptome analysis revealed that 30,311 differentially expressed genes were
activated from leaf bud to mature leaf, and these genes were significantly
enriched in metabolic pathways related to signal transduction and glycosynthesis.
Differential genes were hierarchically clustered into 13 modules. The
correlations of these modules with different growth stages of S. nigrum leaves,
as well as the number and depth of leaf notches were analyzed. It was found that
the turquoise expression module (ME1) was significantly and positively correlated
with the leaf bud stage (r = 0.94, p < 0.01), while negatively correlated with
the number and depth of leaf notches. Three highly connected hub genes were
identified from network interactions analysis of genes related to the leaf
differentiation pathway in Module 1. From the intersection of the turquoise
module and the 2 clusters screened by temporal analysis, the core gene (SnNAC90)
for leaf margin fission in lobelia was identified. The regulatory role of the
SnNAC90 gene in tobacco leaves was preliminarily verified by transgenic
technology. DISCUSSION: It was hypothesized that it might positively regulate
leaf margin fission in S. nigrum. Preliminary speculation on its regulatory role
in S. nigrum leaves. This study, for the first time, revealed the regulatory
mechanism of leaf margin division in S. nigrum, providing a theoretical basis for
enriching its germplasm resources and serving as a reference basis for
cultivating leaf plants.
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