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10.3389/fpls.2025.1711607 http://scihub22266oqcxt.onion/10.3389/fpls.2025.1711607 C12670178!12670178 !41341303     free     free     free       Front+Plant+Sci 2025 ; 16 (?): 1711607 Nephropedia Template TP {{tp|p=41341303 |t=2025. Genome-wide characterisation of the three amino acid loop extension gene family of watermelon in response to abiotic stresses |pdf=|usr=}}{{41341303 }} gab.com Text Genome-wide characterisation of the three amino acid loop extension gene family of watermelon in response to abiotic stresses JO: Front Plant Sci http://www.kidney.de/pget.php?&tw=1&pmid=41341303 #FOAvip INTRODUCTION: The TALE gene family acts as key regulators of plant growth, development, and stress adaptation. However, systematic characterization of this family in watermelon (Citrullus lanatus L.), an economically important cucurbit crop susceptible to abiotic stresses like drought and cold, is lacking. This gap hinders understanding of watermelon's stress-responsive mechanisms and the breeding of stress-resilient varieties. METHODS: ClTALE genes were comprehensively identified using the watermelon genome database. Bioinformatics analyses (phylogenetic classification, genomic structure annotation, conserved motif detection, cis-acting element prediction) were performed. Protein-protein interactions were inferred via STRING. qRT-PCR detected expression profiles under drought, low potassium (LK), and melatonin + cold (MT+CT) treatments. Subcellular localization of candidate genes was analyzed by transient expression, and yeast heterologous expression verified stress tolerance under PEG-simulated drought. RESULTS: A total of 22 ClTALE members were identified, clustering into seven subclades (KNOX-I/STM, KNOX-II, BELL-I to BELL-V). Their promoters contain abundant hormone-related (abscisic acid, jasmonic acid) and abiotic stress-related (drought, cold) cis-acting elements. ClTALE proteins may interact with core growth and development transcription factors. ClTALE2, 3, 8, 11, and 20 were significantly upregulated under drought; ClTALE2 and 3 showed cross-response to LK and MT+CT. ClTALE3 localizes to the nucleus, and its overexpression enhanced yeast tolerance to PEG stress. DISCUSSION: This study is the first systematic characterization of the watermelon ClTALE family, clarifying its genomic features, evolutionary relationships, and stress-responsive patterns. ClTALE2 and 3 (especially ClTALE3) exhibit potential as key stress adaptation regulators. These findings provide a theoretical basis and genetic resources for elucidating watermelon's stress-resistance mechanisms and breeding stress-tolerant varieties. Twit Text FOAVip Genome-wide characterisation of the three amino acid loop extension gene family of watermelon in response to abiotic stresses ????Front Plant Sci http://www.kidney.de/pget.php?&tw=1&pmid=41341303 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=41341303 #FOAvip English Wikipedia <ref>{{Cite pmid|41341303 }}</ref> Genome-wide characterisation of the three amino acid loop extension gene family of watermelon in response to abiotic stresses #MMPMID41341303 Qiu Z ; Dong J ; Chen L ; Zhao L ; Hu L ; Wang H Front Plant Sci 2025[]; 16 (?): 1711607 PMID41341303 show ga INTRODUCTION: The TALE gene family acts as key regulators of plant growth, development, and stress adaptation. However, systematic characterization of this family in watermelon (Citrullus lanatus L.), an economically important cucurbit crop susceptible to abiotic stresses like drought and cold, is lacking. This gap hinders understanding of watermelon's stress-responsive mechanisms and the breeding of stress-resilient varieties. METHODS: ClTALE genes were comprehensively identified using the watermelon genome database. Bioinformatics analyses (phylogenetic classification, genomic structure annotation, conserved motif detection, cis-acting element prediction) were performed. Protein-protein interactions were inferred via STRING. qRT-PCR detected expression profiles under drought, low potassium (LK), and melatonin + cold (MT+CT) treatments. Subcellular localization of candidate genes was analyzed by transient expression, and yeast heterologous expression verified stress tolerance under PEG-simulated drought. RESULTS: A total of 22 ClTALE members were identified, clustering into seven subclades (KNOX-I/STM, KNOX-II, BELL-I to BELL-V). Their promoters contain abundant hormone-related (abscisic acid, jasmonic acid) and abiotic stress-related (drought, cold) cis-acting elements. ClTALE proteins may interact with core growth and development transcription factors. ClTALE2, 3, 8, 11, and 20 were significantly upregulated under drought; ClTALE2 and 3 showed cross-response to LK and MT+CT. ClTALE3 localizes to the nucleus, and its overexpression enhanced yeast tolerance to PEG stress. DISCUSSION: This study is the first systematic characterization of the watermelon ClTALE family, clarifying its genomic features, evolutionary relationships, and stress-responsive patterns. ClTALE2 and 3 (especially ClTALE3) exhibit potential as key stress adaptation regulators. These findings provide a theoretical basis and genetic resources for elucidating watermelon's stress-resistance mechanisms and breeding stress-tolerant varieties. ?Genome-wide characterisation of the three amino acid loop extension ge(epmc).pdf DeepDyve Pubget Overpricing