Analysis of IL-6 and IL-1? Gene coding and study on specific clearance using
histidine-grafted PVDF membranes
#MMPMID41341694
Li K
; Liu J
; Zhang K
; Liu H
; Yang Y
; Na R
; Ma H
; Cui X
Glob Med Genet
2025[Dec]; 12
(4
): 100080
PMID41341694
show ga
Severely infected patients produce large amounts of inflammatory cytokines, such
as interleukin-6 (IL-6) and interleukin-1? (IL-1?), leading to a "cytokine
storm", which is a clinically refractory condition. Because IL-6 and IL-1? differ
significantly in gene family, chromosomal location, protein structure, and
signaling pathways, existing techniques are ineffective at simultaneously
removing cytokines. This work developed a novel blood purification material by
chemically grafting histidine onto a polyvinylidene fluoride (PVDF) membrane.
ATR-FTIR and XPS analyses confirmed the successful grafting of histidine onto the
PVDF membrane. Following histidine grafting, the membrane's adsorption capacity
for IL-6 in aqueous solution increased with histidine concentration. The
optimized histidine-grafted PVDF membrane achieved adsorption rates of (36?±?4)%
for IL-6 and (63?±?5) % for IL-1? in plasma. Additionally, the histidine-grafted
PVDF membrane demonstrated enhanced biocompatibility, exhibiting a low hemolysis
rate, minimal adsorption of red blood cells and platelets, and anticoagulant
properties without activating the coagulation cascade. This histidine-grafted
PVDF membrane offers a promising new therapeutic strategy for treating severe
infections and holds significant potential for clinical application.
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