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2025 ; 25
(1
): 683
Nephropedia Template TP
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English Wikipedia
Endophytic bacterial communities associated with halophytic plants in kebili and
Gabes regions of Southern Tunisia
#MMPMID41131465
Hamdene I
; Bez C
; Bertani I
; López-Menchero JR
; Yahyaoui A
; Venturi V
; Sadfi-Zouaoui N
BMC Microbiol
2025[Oct]; 25
(1
): 683
PMID41131465
show ga
In the arid regions of southern Tunisia, soil and irrigation water salinity
represent major challenges to agricultural sustainability. Despite the increasing
interest in plant-associated microbes, the role of endophytic bacteria in
conferring salt tolerance remains largely unexplored in this context. To address
this gap, twenty-two halophytic plants and their associated soils were sampled
from five distinct sites across the Kebili and Gabes governorates. Significant
differences in soil physicochemical properties were observed between sampling
sites. The soils are generally poorly developed, non-fertile (with very low
organic matter and high CaCO(3) levels), and highly saline, leading to limited
cultivation potential. Molecular identification of plants revealed nine different
families and 14 genera, with the Amaranthaceae family being the most prominent,
including Atriplex spp. (2), Bassia spp. (2), Suaeda spp. (4), and Halocnemum
spp. (1). Bacterial community studies were conducted of both culturable and
non-culturable endophyte communities inhabiting the green and root compartments
of different halotolerant plants. Endophytic microbiome compositions differed
between above-ground and below-ground tissues within the same plant family. A
higher prevalence of three phyla Proteobacteria (67.80%), Firmicutes (14.06%),
and Actinobacteria (6.57%) was detected across all samples. At the genus level,
Acinetobacter, Halomonas, Kushneria, Pseudomonas, Psychrobacter,
Stenotrophomonas, and Streptomyces formed the common core microbiome. Functional
predictions of endophytic bacteria in halophytes highlighted multiple KEGG
functional pathways, indicating recruitment of beneficial bacterial taxa to adapt
to extreme hypersaline conditions, including plant growth-promoting, biocontrol,
and halophilic bacteria.