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Genome-wide identification of BBX gene family and its function in defense of
necrotrophic fungus Alternaria sp in Chrysanthemum
#MMPMID41120830
Wang B
; Liu W
; Gan H
; Lv Y
; Hu X
; Wang T
; Zhao X
BMC Genomics
2025[Oct]; 26
(1
): 942
PMID41120830
show ga
BBX genes play an important role in plant growth, development, and stress
response. However, systematic analysis of BBX gene family regarding resistance to
fungal infections has not been previously conducted in Chrysanthemum. In this
study, a systematic analysis of the BBX gene family was performed, and its
function in defense of necrotrophic fungus Alternaria sp. has been probed into
through publicly available genome and RNA-seq data of Chrysanthemum after
Alternaria sp. infection. The systematic analyses included identifying the BBX
gene family in Chrysanthemum, their evolutionary relationships, conserved
domains, motifs, gene structure, cis-acting elements, and collinearity. Based on
the RNA-seq data analyses and expressional pattern, CmBBX32 was selected as a
candidate gene for further investigation because it responded continuously to the
infection and up-regulated expression when Chrysanthemum was inoculated with
Alternaria sp. Gene expression analysis showed the expression of CmBBX32
increased sharply during the infection process, and was highest in flowers.
Besides, virus-induced gene silencing (VIGS) of CmBBX32 in Chrysanthemum reduced
the resistance to Alternaria sp. infection, as evidenced by phenotypic analysis
of infection symptoms, microscopic examination of spore germination and hyphal
growth, as well as quantitative analysis of the marker gene associated with the
SA and JA defense pathways. Overall, the data generated in this study should form
the basis for future functional characterizations of BBX genes in Chrysanthemum,
especially regarding the resistance to biological stress in Chrysanthemum.
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