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10.1038/s41467-025-63223-6

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suck abstract from ncbi


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pmid40908282
      Nat+Commun 2025 ; 16 (1 ): 8023
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  • Genetic design of soybean hosts and bradyrhizobial endosymbionts reduces N(2)O emissions from soybean rhizosphere #MMPMID40908282
  • Nishida H ; Itakura M ; Win KT ; Li F ; Kakizaki K ; Suzuki A ; Ohkubo S ; Duc LV ; Sugawara M ; Takahashi K ; Shenton M ; Masuda S ; Shibata A ; Shirasu K ; Fujisawa Y ; Tsubokura M ; Akiyama H ; Shimoda Y ; Minamisawa K ; Imaizumi-Anraku H
  • Nat Commun 2025[Sep]; 16 (1 ): 8023 PMID40908282 show ga
  • Soybeans fix atmospheric N(2) through symbiosis with rhizobia. The relationship between rhizobia and soybeans, particularly those with high nitrous oxide (N(2)O)-reducing (N(2)OR) activities, can be leveraged to reduce N(2)O emissions from agricultural soils. However, inoculating soybeans with these rhizobia under field conditions often fails because of the competition from indigenous rhizobia that possess low or no N(2)OR activity. In this work, we utilize natural incompatibility systems between soybean and rhizobia to address this challenge. Specifically, Rj2 and GmNNL1 inhibit certain rhizobial infections in response to NopP, an effector protein. By combining a soybean line with a hybrid accumulation of the Rj2 and GmNNL1 genes and bradyrhizobia lacking the nopP gene, we develop a soybean-bradyrhizobial symbiosis system in which strains with high N(2)OR activity predominantly infect. Our optimize symbiotic system substantially reduces N(2)O emissions in field and laboratory tests, presenting a promising approach for sustainable agricultural practices.
  • |*Bradyrhizobium/genetics/physiology [MESH]
  • |*Glycine max/genetics/microbiology/metabolism [MESH]
  • |*Nitrous Oxide/metabolism [MESH]
  • |*Rhizosphere [MESH]
  • |*Symbiosis/genetics [MESH]
  • |Bacterial Proteins/genetics/metabolism [MESH]
  • |Nitrogen Fixation [MESH]
  • |Rhizobium/genetics [MESH]


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