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10.1186/s12870-025-07292-1

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suck abstract from ncbi


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pmid40908469
      BMC+Plant+Biol 2025 ; 25 (1 ): 1196
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  • Systematic analysis of the Aux/IAA gene family in pineapple reveals insights into their structural diversity, expression dynamics, and interaction networks #MMPMID40908469
  • Wang L ; Sun W ; Jiang W ; Yang Z ; Lin D
  • BMC Plant Biol 2025[Sep]; 25 (1 ): 1196 PMID40908469 show ga
  • BACKGROUND: The Aux/IAA protein is integral to the modulation of auxin signaling, which is essential for plant growth and development. However, systematic analysis on the Aux/IAA gene family in pineapple (Ananas comosus L.) remains unexplored. RESULTS: We systematically identified 20 AcAux/IAA genes in pineapple, classifying them into eight evolutionary groups. Structural analysis revealed conserved domains (I-IV) and intron-exon patterns, with most genes harboring hormone/stress-responsive cis-elements. Subcellular localization confirmed nuclear targeting of all AcIAA-GFP fusion proteins. Tissue-specific expression profiling identified AcIAA1/6/19 as root-enriched, AcIAA2/8 as floral-specific, and AcIAA7 as leaf-preferential. During fruit development, AcIAA9/17?19 peaked at ripening stage, while AcIAA1/3?4/6/11?12/15 declined progressively. Yeast two-hybrid assays uncovered 68 AcIAA-AcARF interactions, with AcARF6/14 showing broad binding capacity, while AcIAA1/17/20 exhibited no interactions. These findings present insights into pineapple Aux/IAA genes, revealing their structural diversity, spatiotemporal expression dynamics, and interaction networks with AcARFs, and highlight their potential roles in organ development and fruit ripening. CONCLUSION: This study establishes a foundation for future functional analyses aimed at elucidating the molecular mechanisms underlying auxin signaling, which governs fruit yield and quality in pineapples. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-025-07292-1.
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