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Virus clearance through apoptosis-dependent phagocytosis of influenza A
virus-infected cells by macrophages
#MMPMID10708457
Fujimoto I
; Pan J
; Takizawa T
; Nakanishi Y
J Virol
2000[Apr]; 74
(7
): 3399-403
PMID10708457
show ga
Some cultured cell lines undergo typical apoptosis upon infection with influenza
virus. However, the release of replicated virus into the culture medium does not
change when apoptosis is inhibited. Since apoptotic cells are heterophagically
eliminated at early stages of the apoptosis pathway, we anticipated that the
coexistence of phagocytic cells with virus-infected cells affects the extent of
virus growth. When influenza A virus-infected HeLa cells were mixed with
activated mouse peritoneal macrophages, efficient phagocytosis, which was
abrogated in the presence of a caspase inhibitor, occurred. At the same time, the
release of virus into the culture medium was completely inhibited, and this
required direct contact between virus-infected cells and macrophages.
Furthermore, an immunoelectron microscopic analysis detected influenza virus
particles associated with phagosome-like structures within macrophages. These
results indicate that apoptosis-dependent phagocytosis of virus-infected cells
may lead to direct elimination of the pathogen.
|*Phagocytosis
[MESH]
|Animals
[MESH]
|Apoptosis/*immunology
[MESH]
|HeLa Cells
[MESH]
|Humans
[MESH]
|Influenza A virus/*immunology/ultrastructure
[MESH]
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