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2000 ; 74
(19
): 9240-4
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Phosphatidylserine-mediated phagocytosis of influenza A virus-infected cells by
mouse peritoneal macrophages
#MMPMID10982371
Shiratsuchi A
; Kaido M
; Takizawa T
; Nakanishi Y
J Virol
2000[Oct]; 74
(19
): 9240-4
PMID10982371
show ga
Influenza virus induces apoptosis in cultured cell lines as well as in animal
tissues. HeLa cells were infected with influenza virus A/Udon/72 (H3N2) under
conditions resulting in almost 100% infection. Such cells underwent typical
caspase-dependent apoptosis and were efficiently phagocytosed by macrophages
prepared from peritoneal fluids of thioglycolate-treated mice. The membrane
phospholipid phosphatidylserine appeared on the surfaces of virus-infected cells
at around the time efficient phagocytosis became detectable. In fact, the
phagocytosis was almost completely inhibited in the presence of liposomes
containing phosphatidylserine, which did not influence the antibody-dependent
uptake of zymosan particles by the same macrophages. These results indicate that
macrophages phagocytose influenza virus-infected HeLa cells in a manner mediated
by phosphatidylserine that appears on the surfaces of infected cells during the
process of apoptosis.