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10.1023/a:1018741312058

http://scihub22266oqcxt.onion/10.1023/a:1018741312058
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9919357!ä!9919357

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suck abstract from ncbi


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pmid9919357      Biochem+Genet 1998 ; 36 (9-10): 311-22
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  • Characterization and expression of the Mx1 gene in wild mouse species #MMPMID9919357
  • Jin HK; Yamashita T; Ochiai K; Haller O; Watanabe T
  • Biochem Genet 1998[Oct]; 36 (9-10): 311-22 PMID9919357show ga
  • The mouse Mx1 gene encodes an interferon (IFN)-inducible nuclear protein and confers resistance to influenza virus infection. The standard laboratory mouse strains all carry the Mx1- allele and are susceptible to influenza virus. In this study, several mouse strains established from wild mice were tested to determine their Mx1+ or Mx1- allele status with polymerase chain reaction-restriction fragment length variation (PCR-RFLV), sequence analysis, reverse transcription (RT)-PCR, and immunofluorescence staining. All of the mouse strains originating from wild mice were found uniformly to carry the Mx1+ allele. Therefore, it is conceivable that the Mx1+ allele in wild populations serves a function against some pathogens related to orthomyxoviruses. The PCR-RFLV and sequence analysis allowed us to classify the Mx1+ alleles of the laboratory and wild-origin mouse strains into distinct classes. RT-PCR and immunofluorescence staining demonstrated that the Mx1 transcripts and proteins were induced by IFN-alpha/beta in macrophages from wild mouse species.
  • |*GTP-Binding Proteins[MESH]
  • |Animals[MESH]
  • |Base Sequence[MESH]
  • |Blotting, Southern[MESH]
  • |Cell Nucleus/metabolism[MESH]
  • |Fluorescent Antibody Technique[MESH]
  • |Interferon-alpha/pharmacology[MESH]
  • |Interferon-beta/pharmacology[MESH]
  • |Macrophages, Peritoneal/metabolism[MESH]
  • |Mice[MESH]
  • |Mice, Inbred BALB C[MESH]
  • |Mice, Inbred C57BL[MESH]
  • |Mice, Inbred CBA[MESH]
  • |Mice, Inbred Strains[MESH]
  • |Molecular Sequence Data[MESH]
  • |Myxovirus Resistance Proteins[MESH]
  • |Orthomyxoviridae/physiology[MESH]
  • |Point Mutation[MESH]
  • |Polymorphism, Restriction Fragment Length[MESH]
  • |Proteins/analysis/*genetics/*physiology[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction[MESH]


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