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10.1016/S0002-9440(10)65727-6 http://scihub22266oqcxt.onion/10.1016/S0002-9440(10)65727-6 9811331/?report=reader!1853394!9811331     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Pathol 1998 ; 153 (5): 1401-9 Nephropedia Template TP {{tp|p=9811331|t=1998. Subtracted, unique-sequence, in situ hybridization: experimental and diagnostic applications |pdf=|usr=}}{{9811331}} gab.com Text Subtracted, unique-sequence, in situ hybridization: experimental and diagnostic applications JO: Am J Pathol http://www.kidney.de/pget.php?&tw=1&pmid=9811331 #FOAvip Nonrandom chromosomal aberrations, particularly in cancer, identify pathogenic biological pathways and, in some cases, have clinical relevance as diagnostic or prognostic markers. Fluorescence and colorimetric in situ hybridization methods facilitate identification of numerical and structural chromosome abnormalities. We report the development of robust, unique-sequence in situ hybridization probes that have several novel features: 1) they are constructed from multimegabase contigs of yeast artificial chromosome (YAC) clones; 2) they are in the form of adapter-ligated, short-fragment, DNA libraries that may be amplified by polymerase chain reaction; and 3) they have had repetitive sequences (eg, Alu and LINE elements) quantitatively removed by subtractive hybridization. These subtracted probes are labeled conveniently, and the fluorescence or colorimetric detection signals are extremely bright. Moreover, they constitute a stable resource that may be amplified through at least four rounds of polymerase chain reaction without diminishing signal intensity. We demonstrate applications of subtracted probes for the MYC and EWS oncogene regions, including 1) characterization of a novel EWS-region translocation in Ewing's sarcoma, 2) identification of chromosomal translocations in paraffin sections, and 3) identification of chromosomal translocations by conventional bright-field microscopy. Twit Text FOAVip Subtracted, unique-sequence, in situ hybridization: experimental and diagnostic applications ????Am J Pathol http://www.kidney.de/pget.php?&tw=1&pmid=9811331 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=9811331 #FOAvip English Wikipedia <ref>{{Cite pmid|9811331}}</ref> Subtracted, unique-sequence, in situ hybridization: experimental and diagnostic applications #MMPMID9811331 Davison JM; Morgan TW; Hsi BL; Xiao S; Fletcher JA Am J Pathol 1998[Nov]; 153 (5): 1401-9 PMID9811331show ga Nonrandom chromosomal aberrations, particularly in cancer, identify pathogenic biological pathways and, in some cases, have clinical relevance as diagnostic or prognostic markers. Fluorescence and colorimetric in situ hybridization methods facilitate identification of numerical and structural chromosome abnormalities. We report the development of robust, unique-sequence in situ hybridization probes that have several novel features: 1) they are constructed from multimegabase contigs of yeast artificial chromosome (YAC) clones; 2) they are in the form of adapter-ligated, short-fragment, DNA libraries that may be amplified by polymerase chain reaction; and 3) they have had repetitive sequences (eg, Alu and LINE elements) quantitatively removed by subtractive hybridization. These subtracted probes are labeled conveniently, and the fluorescence or colorimetric detection signals are extremely bright. Moreover, they constitute a stable resource that may be amplified through at least four rounds of polymerase chain reaction without diminishing signal intensity. We demonstrate applications of subtracted probes for the MYC and EWS oncogene regions, including 1) characterization of a novel EWS-region translocation in Ewing's sarcoma, 2) identification of chromosomal translocations in paraffin sections, and 3) identification of chromosomal translocations by conventional bright-field microscopy. |Burkitt Lymphoma/genetics[MESH] |Chromosome Mapping[MESH] |Chromosomes, Artificial, Yeast[MESH] |Chromosomes, Human, Pair 22[MESH] |Chromosomes, Human, Pair 8[MESH] |Colorimetry[MESH] |Gene Library[MESH] |Genes, myc/*genetics[MESH] |Heterogeneous-Nuclear Ribonucleoproteins[MESH] |Humans[MESH] |In Situ Hybridization/*methods[MESH] |Polymerase Chain Reaction[MESH] |RNA-Binding Protein EWS[MESH] |Repetitive Sequences, Nucleic Acid[MESH] |Ribonucleoproteins/genetics[MESH] |Spectrometry, Fluorescence[MESH]Subtracted, unique-sequence, in situ hybridization: experimental and d(epmc).pdf DeepDyve Pubget Overpricing