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Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 J+Vet+Med+Sci 1998 ; 60 (4): 531-4 Nephropedia Template TP
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Evaluation of serological diagnosis of Borna disease virus infection using recombinant proteins in experimentally infected rats #MMPMID9592731
Ogino M; Yoshimatsu K; Tsujimura K; Mizutani T; Arikawa J; Takashima I
J Vet Med Sci 1998[Apr]; 60 (4): 531-4 PMID9592731show ga
We produced two recombinant Borna disease virus (BDV) proteins, p40 and p24, by using a baculovirus vector as a diagnostic antigen. Antigenicities of these recombinant proteins were evaluated by immune rabbit sera. Recombinant p40 was a more sensitive antigen than p24 for the detection of antibodies in infected rats. Rats inoculated with BDV within 24 hr after birth showed higher detection rates of viral RNA and viral proteins from the brain than rats inoculated at 4 weeks-old. Depending on the age of infection and the time postinfection, the detection of BDV RNA, protein, or anti-BDV antibody did not always correlate in individuals. We suggest both serological and molecular biological methods are needed in the diagnosis of BDV infection.