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  • Evaluation of serological diagnosis of Borna disease virus infection using recombinant proteins in experimentally infected rats #MMPMID9592731
  • Ogino M; Yoshimatsu K; Tsujimura K; Mizutani T; Arikawa J; Takashima I
  • J Vet Med Sci 1998[Apr]; 60 (4): 531-4 PMID9592731garesp_yesshow ga
  • We produced two recombinant Borna disease virus (BDV) proteins, p40 and p24, by using a baculovirus vector as a diagnostic antigen. Antigenicities of these recombinant proteins were evaluated by immune rabbit sera. Recombinant p40 was a more sensitive antigen than p24 for the detection of antibodies in infected rats. Rats inoculated with BDV within 24 hr after birth showed higher detection rates of viral RNA and viral proteins from the brain than rats inoculated at 4 weeks-old. Depending on the age of infection and the time postinfection, the detection of BDV RNA, protein, or anti-BDV antibody did not always correlate in individuals. We suggest both serological and molecular biological methods are needed in the diagnosis of BDV infection.
  • |*Antigens, Viral[MESH]
  • |*Viral Proteins[MESH]
  • |Animals[MESH]
  • |Antibodies, Viral/*blood[MESH]
  • |Borna Disease/blood/*diagnosis[MESH]
  • |Borna disease virus/*isolation & purification[MESH]
  • |Brain/virology[MESH]
  • |Cell Line[MESH]
  • |DNA Primers[MESH]
  • |Polymerase Chain Reaction[MESH]
  • |RNA, Viral/analysis[MESH]
  • |Rabbits[MESH]
  • |Rats[MESH]
  • |Recombinant Proteins[MESH]

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  • garesp_yesshow ga

    531 4.60 1998