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10.1172/JCI686 http://scihub22266oqcxt.onion/10.1172/JCI686 9486975!508656!9486975     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Invest 1998 ; 101 (5): 1045-54 Nephropedia Template TP {{tp|p=9486975|t=1998. Potassium depletion downregulates chloride-absorbing transporters in rat kidney |pdf=|usr=}}{{9486975}} gab.com Text Potassium depletion downregulates chloride-absorbing transporters in rat kidney JO: J Clin Invest http://www.kidney.de/pget.php?&tw=1&pmid=9486975 #FOAvip Potassium depletion (KD) causes renal chloride wasting, suggesting defect(s) in Cl- reabsorption in renal tubules. To determine whether alterations in expression of the major Cl- transporter genes might contribute to the chloride wasting, we analyzed their expression in renal cortex and medulla of animals placed on KD diet. Feeding KD diet to rats resulted in significant hypokalemia at 14 d but not at 6 d. Northern hybridization revealed that mRNA levels for the apical Na-K-2Cl cotransporter in the medulla decreased by 56 and 51% at 6 and 14 d of KD diet, respectively. Functional studies in tubular suspensions from medullary thick ascending limb demonstrated that the Na-K-2Cl cotransporter activity decreased by approximately 45 and approximately 37% at 6 and 14 d of KD diet, respectively. mRNA levels for the thiazide-sensitive Na-Cl cotransporter decreased by 57 and 64% at 6 and 14 d of KD diet. Decreased expression of the apical Na-Cl and the Na-K-2Cl cotransporters became evident at 48 and 72 h of KD, respectively. Urinary chloride excretion increased at 48 h and further increased at 72 h of KD, correlating with suppression of the Na-Cl and the Na-K-2Cl transporters. Our results indicate that increased urinary chloride loss in KD results from suppression of the chloride-absorbing transporters. Downregulation of chloride transporters in KD is an early event and can lead to hypochloremia and subsequently hypovolemia and decreased glomerular filtration rate. Twit Text FOAVip Potassium depletion downregulates chloride-absorbing transporters in rat kidney ????J Clin Invest http://www.kidney.de/pget.php?&tw=1&pmid=9486975 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=9486975 #FOAvip English Wikipedia <ref>{{Cite pmid|9486975}}</ref> Potassium depletion downregulates chloride-absorbing transporters in rat kidney #MMPMID9486975 Amlal H; Wang Z; Soleimani M J Clin Invest 1998[Mar]; 101 (5): 1045-54 PMID9486975show ga Potassium depletion (KD) causes renal chloride wasting, suggesting defect(s) in Cl- reabsorption in renal tubules. To determine whether alterations in expression of the major Cl- transporter genes might contribute to the chloride wasting, we analyzed their expression in renal cortex and medulla of animals placed on KD diet. Feeding KD diet to rats resulted in significant hypokalemia at 14 d but not at 6 d. Northern hybridization revealed that mRNA levels for the apical Na-K-2Cl cotransporter in the medulla decreased by 56 and 51% at 6 and 14 d of KD diet, respectively. Functional studies in tubular suspensions from medullary thick ascending limb demonstrated that the Na-K-2Cl cotransporter activity decreased by approximately 45 and approximately 37% at 6 and 14 d of KD diet, respectively. mRNA levels for the thiazide-sensitive Na-Cl cotransporter decreased by 57 and 64% at 6 and 14 d of KD diet. Decreased expression of the apical Na-Cl and the Na-K-2Cl cotransporters became evident at 48 and 72 h of KD, respectively. Urinary chloride excretion increased at 48 h and further increased at 72 h of KD, correlating with suppression of the Na-Cl and the Na-K-2Cl transporters. Our results indicate that increased urinary chloride loss in KD results from suppression of the chloride-absorbing transporters. Downregulation of chloride transporters in KD is an early event and can lead to hypochloremia and subsequently hypovolemia and decreased glomerular filtration rate. |*Symporters[MESH] |Animals[MESH] |Benzothiadiazines[MESH] |Biological Transport, Active/genetics[MESH] |Blotting, Northern[MESH] |Carrier Proteins/genetics/*metabolism[MESH] |Chlorides/*metabolism[MESH] |Diuretics[MESH] |Down-Regulation[MESH] |Gene Expression[MESH] |Hypokalemia/metabolism[MESH] |Ion Transport/genetics[MESH] |Kidney Cortex/metabolism[MESH] |Kidney Diseases/*metabolism[MESH] |Kidney Medulla/metabolism/physiology[MESH] |Kidney/physiopathology[MESH] |Male[MESH] |Potassium Deficiency/*metabolism[MESH] |RNA, Messenger/analysis/metabolism[MESH] |Rats[MESH] |Rats, Sprague-Dawley[MESH] |Sodium Chloride Symporter Inhibitors/pharmacology[MESH] |Sodium Chloride Symporters[MESH]Potassium depletion downregulates chloride-absorbing transporters in r(epmc).pdf DeepDyve Pubget Overpricing