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10.1085/jgp.111.1.139 http://scihub22266oqcxt.onion/10.1085/jgp.111.1.139 9417141!1887768!9417141     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Gen+Physiol 1998 ; 111 (1): 139-60 Nephropedia Template TP {{tp|p=9417141|t=1998. Properties of an inwardly rectifying ATP-sensitive K+ channel in the basolateral membrane of renal proximal tubule |pdf=|usr=}}{{9417141}} gab.com Text Properties of an inwardly rectifying ATP-sensitive K+ channel in the basolateral membrane of renal proximal tubule JO: J Gen Physiol http://www.kidney.de/pget.php?&tw=1&pmid=9417141 #FOAvip The potassium conductance of the basolateral membrane (BLM) of proximal tubule cells is a critical regulator of transport since it is the major determinant of the negative cell membrane potential and is necessary for pump-leak coupling to the Na+,K+-ATPase pump. Despite this pivotal physiological role, the properties of this conductance have been incompletely characterized, in part due to difficulty gaining access to the BLM. We have investigated the properties of this BLM K+ conductance in dissociated, polarized Ambystoma proximal tubule cells. Nearly all seals made on Ambystoma cells contained inward rectifier K+ channels (gammaslope, in = 24.5 +/- 0.6 pS, gammachord, out = 3.7 +/- 0.4 pS). The rectification is mediated in part by internal Mg2+. The open probability of the channel increases modestly with hyperpolarization. The inward conducting properties are described by a saturating binding-unbinding model. The channel conducts Tl+ and K+, but there is no significant conductance for Na+, Rb+, Cs+, Li+, NH4+, or Cl-. The channel is inhibited by barium and the sulfonylurea agent glibenclamide, but not by tetraethylammonium. Channel rundown typically occurs in the absence of ATP, but cytosolic addition of 0. 2 mM ATP (or any hydrolyzable nucleoside triphosphate) sustains channel activity indefinitely. Phosphorylation processes alone fail to sustain channel activity. Higher doses of ATP (or other nucleoside triphosphates) reversibly inhibit the channel. The K+ channel opener diazoxide opens the channel in the presence of 0.2 mM ATP, but does not alleviate the inhibition of millimolar doses of ATP. We conclude that this K+ channel is the major ATP-sensitive basolateral K+ conductance in the proximal tubule. Twit Text FOAVip Properties of an inwardly rectifying ATP-sensitive K+ channel in the basolateral membrane of renal proximal tubule ????J Gen Physiol http://www.kidney.de/pget.php?&tw=1&pmid=9417141 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=9417141 #FOAvip English Wikipedia <ref>{{Cite pmid|9417141}}</ref> Properties of an inwardly rectifying ATP-sensitive K+ channel in the basolateral membrane of renal proximal tubule #MMPMID9417141 Mauerer UR; Boulpaep EL; Segal AS J Gen Physiol 1998[Jan]; 111 (1): 139-60 PMID9417141show ga The potassium conductance of the basolateral membrane (BLM) of proximal tubule cells is a critical regulator of transport since it is the major determinant of the negative cell membrane potential and is necessary for pump-leak coupling to the Na+,K+-ATPase pump. Despite this pivotal physiological role, the properties of this conductance have been incompletely characterized, in part due to difficulty gaining access to the BLM. We have investigated the properties of this BLM K+ conductance in dissociated, polarized Ambystoma proximal tubule cells. Nearly all seals made on Ambystoma cells contained inward rectifier K+ channels (gammaslope, in = 24.5 +/- 0.6 pS, gammachord, out = 3.7 +/- 0.4 pS). The rectification is mediated in part by internal Mg2+. The open probability of the channel increases modestly with hyperpolarization. The inward conducting properties are described by a saturating binding-unbinding model. The channel conducts Tl+ and K+, but there is no significant conductance for Na+, Rb+, Cs+, Li+, NH4+, or Cl-. The channel is inhibited by barium and the sulfonylurea agent glibenclamide, but not by tetraethylammonium. Channel rundown typically occurs in the absence of ATP, but cytosolic addition of 0. 2 mM ATP (or any hydrolyzable nucleoside triphosphate) sustains channel activity indefinitely. Phosphorylation processes alone fail to sustain channel activity. Higher doses of ATP (or other nucleoside triphosphates) reversibly inhibit the channel. The K+ channel opener diazoxide opens the channel in the presence of 0.2 mM ATP, but does not alleviate the inhibition of millimolar doses of ATP. We conclude that this K+ channel is the major ATP-sensitive basolateral K+ conductance in the proximal tubule. |Adenosine Triphosphate/*metabolism/pharmacology[MESH] |Ambystoma[MESH] |Animals[MESH] |Barium/pharmacology[MESH] |Biological Transport/drug effects/physiology[MESH] |Cations/metabolism[MESH] |Cells, Cultured[MESH] |Cytidine Triphosphate/pharmacology[MESH] |Diazoxide/pharmacology[MESH] |Diuretics[MESH] |Electric Conductivity[MESH] |Glyburide/pharmacology[MESH] |Guanosine Triphosphate/pharmacology[MESH] |Hypoglycemic Agents/pharmacology[MESH] |Inosine Triphosphate/pharmacology[MESH] |Ion Channel Gating/drug effects/*physiology[MESH] |Kidney Tubules, Proximal/*chemistry/cytology[MESH] |Kinetics[MESH] |Microvilli/chemistry/enzymology[MESH] |Patch-Clamp Techniques[MESH] |Potassium Channels/*physiology[MESH] |Sodium Chloride Symporter Inhibitors/pharmacology[MESH] |Sodium-Potassium-Exchanging ATPase/metabolism[MESH] |Thallium[MESH] |Thymine Nucleotides/pharmacology[MESH]Properties of an inwardly rectifying ATP-sensitive K+ channel in the b(epmc).pdf DeepDyve Pubget Overpricing