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10.1085/jgp.110.6.643 http://scihub22266oqcxt.onion/10.1085/jgp.110.6.643 9382893!2229399!9382893     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Gen+Physiol 1997 ; 110 (6): 643-54 Nephropedia Template TP {{tp|p=9382893|t=1997. Regulation of KATP channel activity by diazoxide and MgADP Distinct functions of the two nucleotide binding folds of the sulfonylurea receptor |pdf=|usr=}}{{9382893}} gab.com Text Regulation of KATP channel activity by diazoxide and MgADP Distinct functions of the two nucleotide binding folds of the sulfonylurea receptor JO: J Gen Physiol http://www.kidney.de/pget.php?&tw=1&pmid=9382893 #FOAvip KATP channels were reconstituted in COSm6 cells by coexpression of the sulfonylurea receptor SUR1 and the inward rectifier potassium channel Kir6.2. The role of the two nucleotide binding folds of SUR1 in regulation of KATP channel activity by nucleotides and diazoxide was investigated. Mutations in the linker region and the Walker B motif (Walker, J.E., M.J. Saraste, M.J. Runswick, and N.J. Gay. 1982. EMBO [Eur. Mol. Biol. Organ.] J. 1:945-951) of the second nucleotide binding fold, including G1479D, G1479R, G1485D, G1485R, Q1486H, and D1506A, all abolished stimulation by MgADP and diazoxide, with the exception of G1479R, which showed a small stimulatory response to diazoxide. Analogous mutations in the first nucleotide binding fold, including G827D, G827R, and Q834H, were still stimulated by diazoxide and MgADP, but with altered kinetics compared with the wild-type channel. None of the mutations altered the sensitivity of the channel to inhibition by ATP4-. We propose a model in which SUR1 sensitizes the KATP channel to ATP inhibition, and nucleotide hydrolysis at the nucleotide binding folds blocks this effect. MgADP and diazoxide are proposed to stabilize this desensitized state of the channel, and mutations at the nucleotide binding folds alter the response of channels to MgADP and diazoxide by altering nucleotide hydrolysis rates or the coupling of hydrolysis to channel activation. Twit Text FOAVip Regulation of KATP channel activity by diazoxide and MgADP Distinct functions of the two nucleotide binding folds of the sulfonylurea receptor ????J Gen Physiol http://www.kidney.de/pget.php?&tw=1&pmid=9382893 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=9382893 #FOAvip English Wikipedia <ref>{{Cite pmid|9382893}}</ref> Regulation of KATP channel activity by diazoxide and MgADP Distinct functions of the two nucleotide binding folds of the sulfonylurea receptor #MMPMID9382893 Shyng S; Ferrigni T; Nichols CG J Gen Physiol 1997[Dec]; 110 (6): 643-54 PMID9382893show ga KATP channels were reconstituted in COSm6 cells by coexpression of the sulfonylurea receptor SUR1 and the inward rectifier potassium channel Kir6.2. The role of the two nucleotide binding folds of SUR1 in regulation of KATP channel activity by nucleotides and diazoxide was investigated. Mutations in the linker region and the Walker B motif (Walker, J.E., M.J. Saraste, M.J. Runswick, and N.J. Gay. 1982. EMBO [Eur. Mol. Biol. Organ.] J. 1:945-951) of the second nucleotide binding fold, including G1479D, G1479R, G1485D, G1485R, Q1486H, and D1506A, all abolished stimulation by MgADP and diazoxide, with the exception of G1479R, which showed a small stimulatory response to diazoxide. Analogous mutations in the first nucleotide binding fold, including G827D, G827R, and Q834H, were still stimulated by diazoxide and MgADP, but with altered kinetics compared with the wild-type channel. None of the mutations altered the sensitivity of the channel to inhibition by ATP4-. We propose a model in which SUR1 sensitizes the KATP channel to ATP inhibition, and nucleotide hydrolysis at the nucleotide binding folds blocks this effect. MgADP and diazoxide are proposed to stabilize this desensitized state of the channel, and mutations at the nucleotide binding folds alter the response of channels to MgADP and diazoxide by altering nucleotide hydrolysis rates or the coupling of hydrolysis to channel activation. |*ATP-Binding Cassette Transporters[MESH] |*Potassium Channels, Inwardly Rectifying[MESH] |Adenosine Diphosphate/*pharmacology[MESH] |Adenosine Triphosphate/pharmacology[MESH] |Animals[MESH] |Antihypertensive Agents/*pharmacology[MESH] |COS Cells/chemistry/physiology[MESH] |Diazoxide/*pharmacology[MESH] |Hydrolysis[MESH] |Ion Channel Gating/drug effects[MESH] |Kinetics[MESH] |Magnesium/pharmacology[MESH] |Mutagenesis, Site-Directed/physiology[MESH] |Patch-Clamp Techniques[MESH] |Potassium Channels/chemistry/*genetics/metabolism[MESH] |Protein Folding[MESH] |Receptors, Drug/chemistry/*genetics/metabolism[MESH] |Sulfonylurea Compounds/chemistry/metabolism[MESH]Regulation of KATP channel activity by diazoxide and MgADP Distinct f(epmc).pdf DeepDyve Pubget Overpricing