Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText.
Kick-your-searchterm to multiple Engines kick-your-query now !>
A dictionary by aggregated review articles of nephrology, medicine and the life sciences
Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

suck abstract from ncbi


10.1099/0022-1317-78-10-2459

http://scihub22266oqcxt.onion/10.1099/0022-1317-78-10-2459
suck pdf from google scholar
9349465!ä!9349465

suck abstract from ncbi


Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
pmid9349465      J+Gen+Virol 1997 ; 78 ( Pt 10) (ä): 2459-66
Nephropedia Template TP

gab.com Text

Twit Text FOAVip

Twit Text #

English Wikipedia


  • Detection of a novel Borna disease virus-encoded 10 kDa protein in infected cells and tissues #MMPMID9349465
  • Wehner T; Ruppert A; Herden C; Frese K; Becht H; Richt JA
  • J Gen Virol 1997[Oct]; 78 ( Pt 10) (ä): 2459-66 PMID9349465show ga
  • Borna disease (BD) is a transmissible, progressive polioencephalomyelitis primarily of horses and sheep. The genomes of two cell-adapted strains of Borna disease virus (BDV), the aetiological agent of BD, have been cloned and sequenced. According to the structural characterization achieved so far, BDV contains a non-segmented negative-sense 8.9 kb single-stranded RNA genome. In this paper we report the expression, purification and intracellular tracing of a novel non-glycosylated BDV-specific protein with a molecular mass of approximately 10 kDa (BDV p10 protein). The successful isolation of the corresponding mRNA from infected cells, amplification of the genetic region by RT-PCR and its efficient expression as a glutathione S-transferase (GST) fusion protein demonstrated that antibodies specific for the BDV p10 protein are induced in infected animals. In addition, we have produced monospecific antisera against the GST-p10 fusion protein in rabbits. This monospecific antiserum recognized the BDV p10 protein in brain cells of naturally and experimentally infected animals as well as in persistently BDV-infected cells. Antibody-mediated affinity-chromatography using the anti-p10 serum could successfully be applied to purify a ca. 10 kDa antigen from infected animal cells to such an extent that glycosylation of this component could be ruled out.
  • |Animals[MESH]
  • |Antibodies, Viral/immunology[MESH]
  • |Base Sequence[MESH]
  • |Borna Disease/*virology[MESH]
  • |Borna disease virus/*chemistry[MESH]
  • |Cells, Cultured[MESH]
  • |Cloning, Molecular[MESH]
  • |Dogs[MESH]
  • |Genes, Viral[MESH]
  • |Molecular Sequence Data[MESH]
  • |Molecular Weight[MESH]
  • |Open Reading Frames[MESH]
  • |RNA, Viral/genetics[MESH]
  • |Rabbits[MESH]
  • |Rats[MESH]
  • |Recombinant Fusion Proteins[MESH]
  • |Viral Proteins/*analysis/immunology/isolation & purification[MESH]


  • DeepDyve
  • Pubget Overpricing
  • suck abstract from ncbi

    Linkout box