Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1074/jbc.272.35.21818 http://scihub22266oqcxt.onion/10.1074/jbc.272.35.21818 9268312!ä!9268312 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=9268312&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Biol+Chem 1997 ; 272 (35): 21818-23 Nephropedia Template TP {{tp|p=9268312|t=1997. Borna disease virus P-protein is phosphorylated by protein kinase Cepsilon and casein kinase II |pdf=|usr=}}{{9268312}} gab.com Text Borna disease virus P-protein is phosphorylated by protein kinase Cepsilon and casein kinase II JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=9268312 #FOAvip Borna disease virus (BDV) is a newly classified nonsegmented negative-strand RNA virus (order of Mononegavirales) that persistently infects specific brain regions and circuits of warm-blooded animals to cause behavioral disturbances. Viruses within the order of Mononegavirales have phosphoproteins that typically serve as transcription factors and are modulated in functional activity through phosphorylation. To identify the kinases involved in BDV phosphoprotein (BDV-P) phosphorylation, in vitro phosphorylation assays were performed using recombinant phosphoprotein produced in Escherichia coli as substrate and cytoplasmic extracts from a rat glioma cell line (C6) or rat brain extracts as sources of kinase activity. These experiments revealed that BDV-P was phosphorylated predominantly by protein kinase C (PKC) and to a lesser extent by casein kinase II. Partial purification of the PKC from rat brain extract suggested that the BDV-P phosphorylating kinase is PKCepsilon. A role for PKC phosphorylation in vivo was confirmed by using the PKC-specific inhibitor GF109203X. Furthermore, peptide mapping studies indicated that BDV-P is phosphorylated at the same sites in vitro as it is in vivo. Mutational analysis identified Ser26 and Ser28 as sites for PKC phosphorylation and Ser70 and Ser86 as sites for casein kinase II phosphorylation. The anatomic distribution of PKCepsilon in the central nervous system may have implications for BDV neurotropism and pathogenesis. Twit Text FOAVip Borna disease virus P-protein is phosphorylated by protein kinase Cepsilon and casein kinase II ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=9268312 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=9268312 #FOAvip English Wikipedia <ref>{{Cite pmid|9268312}}</ref> Borna disease virus P-protein is phosphorylated by protein kinase Cepsilon and casein kinase II #MMPMID9268312 Schwemmle M; De B; Shi L; Banerjee A; Lipkin WI J Biol Chem 1997[Aug]; 272 (35): 21818-23 PMID9268312show ga Borna disease virus (BDV) is a newly classified nonsegmented negative-strand RNA virus (order of Mononegavirales) that persistently infects specific brain regions and circuits of warm-blooded animals to cause behavioral disturbances. Viruses within the order of Mononegavirales have phosphoproteins that typically serve as transcription factors and are modulated in functional activity through phosphorylation. To identify the kinases involved in BDV phosphoprotein (BDV-P) phosphorylation, in vitro phosphorylation assays were performed using recombinant phosphoprotein produced in Escherichia coli as substrate and cytoplasmic extracts from a rat glioma cell line (C6) or rat brain extracts as sources of kinase activity. These experiments revealed that BDV-P was phosphorylated predominantly by protein kinase C (PKC) and to a lesser extent by casein kinase II. Partial purification of the PKC from rat brain extract suggested that the BDV-P phosphorylating kinase is PKCepsilon. A role for PKC phosphorylation in vivo was confirmed by using the PKC-specific inhibitor GF109203X. Furthermore, peptide mapping studies indicated that BDV-P is phosphorylated at the same sites in vitro as it is in vivo. Mutational analysis identified Ser26 and Ser28 as sites for PKC phosphorylation and Ser70 and Ser86 as sites for casein kinase II phosphorylation. The anatomic distribution of PKCepsilon in the central nervous system may have implications for BDV neurotropism and pathogenesis. |Animals[MESH] |Borna disease virus/*metabolism[MESH] |Calcium/metabolism[MESH] |Casein Kinase II[MESH] |Cell Line[MESH] |Enzyme Inhibitors/pharmacology[MESH] |Heparin/metabolism[MESH] |Indoles/pharmacology[MESH] |Isoenzymes/antagonists & inhibitors/*metabolism[MESH] |Maleimides/pharmacology[MESH] |Phosphoproteins/*metabolism[MESH] |Phosphorylation[MESH] |Protein Kinase C-epsilon[MESH] |Protein Kinase C/antagonists & inhibitors/*metabolism[MESH] |Protein Serine-Threonine Kinases/*metabolism[MESH] |Rats[MESH]Borna disease virus P-protein is phosphorylated by protein kinase Ceps(epmc).pdf DeepDyve Pubget Overpricing