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10.1111/j.1348-0421.1997.tb01881.x http://scihub22266oqcxt.onion/10.1111/j.1348-0421.1997.tb01881.x 9251059!ä!9251059 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Microbiol+Immunol 1997 ; 41 (6): 481-6 Nephropedia Template TP {{tp|p=9251059|t=1997. Amplification of a full-length Borna disease virus (BDV) cDNA from total RNA of cells persistently infected with BDV |pdf=|usr=}}{{9251059}} gab.com Text Amplification of a full-length Borna disease virus (BDV) cDNA from total RNA of cells persistently infected with BDV JO: Microbiol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=9251059 #FOAvip We have developed a novel reverse transcriptase-polymerase chain reaction (RT-PCR) to amplify the full-length 8.9 kilobase (kbp) cDNA of the Borna disease virus (BDV) RNA genome from the total cellular RNA of MDCK cells persistently infected with BDV (MDCK/BDV). Antigenomic BDV cDNA was reverse transcribed using a 53-mer oligonucleotide primer, corresponding to the 5'-terminus of a putative 3'-leader sequence of the BDV RNA genome, for 2 hr at 42 C followed by 30 min at 55 C. PCR was performed in the presence of this 53-mer antigenomic primer and a 25-mer primer, corresponding to the 3'-terminus of the BDV antigenomic cDNA, by use of an rTth DNA polymerase with proof-reading activity. The amplified full-length BDV cDNA was detected in as little as 20 ng of total cellular RNA of MDCK/BDV. This RT-PCR method should be a useful technique to study the molecular quasispecies of BDV. Twit Text FOAVip Amplification of a full-length Borna disease virus (BDV) cDNA from total RNA of cells persistently infected with BDV ????Microbiol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=9251059 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=9251059 #FOAvip English Wikipedia <ref>{{Cite pmid|9251059}}</ref> Amplification of a full-length Borna disease virus (BDV) cDNA from total RNA of cells persistently infected with BDV #MMPMID9251059 Shoya Y; Kobayashi T; Koda T; Lai PK; Tanaka H; Koyama T; Ikuta K; Kakinuma M; Kishi M Microbiol Immunol 1997[]; 41 (6): 481-6 PMID9251059show ga We have developed a novel reverse transcriptase-polymerase chain reaction (RT-PCR) to amplify the full-length 8.9 kilobase (kbp) cDNA of the Borna disease virus (BDV) RNA genome from the total cellular RNA of MDCK cells persistently infected with BDV (MDCK/BDV). Antigenomic BDV cDNA was reverse transcribed using a 53-mer oligonucleotide primer, corresponding to the 5'-terminus of a putative 3'-leader sequence of the BDV RNA genome, for 2 hr at 42 C followed by 30 min at 55 C. PCR was performed in the presence of this 53-mer antigenomic primer and a 25-mer primer, corresponding to the 3'-terminus of the BDV antigenomic cDNA, by use of an rTth DNA polymerase with proof-reading activity. The amplified full-length BDV cDNA was detected in as little as 20 ng of total cellular RNA of MDCK/BDV. This RT-PCR method should be a useful technique to study the molecular quasispecies of BDV. |Animals[MESH] |Borna disease virus/*genetics[MESH] |Cells, Cultured[MESH] |DNA, Complementary/*genetics[MESH] |DNA, Viral/*genetics[MESH] |Dogs[MESH] |Molecular Sequence Data[MESH] |Polymerase Chain Reaction/*methods[MESH] |RNA, Viral/*genetics[MESH]Amplification of a full-length Borna disease virus (BDV) cDNA from tot(epmc).pdf DeepDyve Pubget Overpricing