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10.1152/ajprenal.1997.272.6.F759 http://scihub22266oqcxt.onion/10.1152/ajprenal.1997.272.6.F759 9227637!ä!9227637 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Physiol 1997 ; 272 (6 Pt 2): F759-66 Nephropedia Template TP {{tp|p=9227637|t=1997. Acid-base changes alter Mg2+ uptake in mouse distal convoluted tubule cells |pdf=|usr=}}{{9227637}} gab.com Text Acid-base changes alter Mg2+ uptake in mouse distal convoluted tubule cells JO: Am J Physiol http://www.kidney.de/pget.php?&tw=1&pmid=9227637 #FOAvip Metabolic alkalosis leads to renal magnesium conservation, whereas metabolic acidosis is associated with urinary magnesium wasting. Micropuncture studies suggest that these actions affect magnesium transport in the distal tubule. The cellular mechanisms of acid-base changes were investigated in an immortalized mouse distal convoluted tubule (MDCT) cell line. Intracellular free Mg2+ concentration ([Mg2+]i) was determined by microfluorescence using the Mg(2+)-responsive dye, mag-fura 2. Mg2+ transport was assessed as a function of change in [Mg2+]i with time following placement of Mg(2+)-depleted cells into a buffer containing 1.5 mM magnesium. The uptake rate of Mg2+, d([Mg2+]i)/dt, into Mg(2+)-depleted cells determined with a buffer solution of pH 7.4 was 178 +/- 21 nM/s. Mg2+ uptake at pH 8.0 was markedly increased 278 +/- 35 nM/s, whereas transport at pH 6.0 was significantly reduced to 121 +/- 15 nM/s. Mg2+ uptake at pH 7.4 was not stimulated with 20 or 40 mM bicarbonate, nor were the differences in Mg2+ uptake with pH associated with changes in membrane voltage. Mg2+ uptake was stimulated with membrane hyperpolarization at pH 6.0 but not at pH 8.0. Chlorothiazide (10(-4) M), which stimulates Mg2+ uptake by hyperpolarizing the membrane voltage, increased uptake at pH 6.0, 59 +/- 14%, but decreased it at alkaline pH of 8.0, -55 +/- 3%. Accordingly, MDCT cells become refractory to the stimulating effects of hyperpolarization at alkaline pH values. These studies show that protons may directly affect Mg2+ transport in MDCT cells. Twit Text FOAVip Acid-base changes alter Mg2+ uptake in mouse distal convoluted tubule cells ????Am J Physiol http://www.kidney.de/pget.php?&tw=1&pmid=9227637 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=9227637 #FOAvip English Wikipedia <ref>{{Cite pmid|9227637}}</ref> Acid-base changes alter Mg2+ uptake in mouse distal convoluted tubule cells #MMPMID9227637 Dai LJ; Friedman PA; Quamme GA Am J Physiol 1997[Jun]; 272 (6 Pt 2): F759-66 PMID9227637show ga Metabolic alkalosis leads to renal magnesium conservation, whereas metabolic acidosis is associated with urinary magnesium wasting. Micropuncture studies suggest that these actions affect magnesium transport in the distal tubule. The cellular mechanisms of acid-base changes were investigated in an immortalized mouse distal convoluted tubule (MDCT) cell line. Intracellular free Mg2+ concentration ([Mg2+]i) was determined by microfluorescence using the Mg(2+)-responsive dye, mag-fura 2. Mg2+ transport was assessed as a function of change in [Mg2+]i with time following placement of Mg(2+)-depleted cells into a buffer containing 1.5 mM magnesium. The uptake rate of Mg2+, d([Mg2+]i)/dt, into Mg(2+)-depleted cells determined with a buffer solution of pH 7.4 was 178 +/- 21 nM/s. Mg2+ uptake at pH 8.0 was markedly increased 278 +/- 35 nM/s, whereas transport at pH 6.0 was significantly reduced to 121 +/- 15 nM/s. Mg2+ uptake at pH 7.4 was not stimulated with 20 or 40 mM bicarbonate, nor were the differences in Mg2+ uptake with pH associated with changes in membrane voltage. Mg2+ uptake was stimulated with membrane hyperpolarization at pH 6.0 but not at pH 8.0. Chlorothiazide (10(-4) M), which stimulates Mg2+ uptake by hyperpolarizing the membrane voltage, increased uptake at pH 6.0, 59 +/- 14%, but decreased it at alkaline pH of 8.0, -55 +/- 3%. Accordingly, MDCT cells become refractory to the stimulating effects of hyperpolarization at alkaline pH values. These studies show that protons may directly affect Mg2+ transport in MDCT cells. |*Acid-Base Equilibrium[MESH] |Animals[MESH] |Cell Line, Transformed[MESH] |Chlorothiazide/pharmacology[MESH] |Electrophysiology[MESH] |Hydrogen-Ion Concentration[MESH] |Kidney Tubules, Distal/cytology/*metabolism/physiology[MESH] |Magnesium/antagonists & inhibitors/*pharmacokinetics[MESH] |Membranes/physiology[MESH] |Mice[MESH] |Nifedipine/pharmacology[MESH]Acid-base changes alter Mg2+ uptake in mouse distal convoluted tubule (epmc).pdf DeepDyve Pubget Overpricing